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目的观察含氯消毒剂对耐药结核分枝杆菌的杀灭效果,检测其耐消毒剂基因。方法采用悬液定量杀菌试验和基因扩增法,对该含氯消毒剂杀灭结核分枝杆菌的效果进行了观察和基因检测。结果用含有效氯1 000mg/L的含氯消毒剂作用10 min,对悬液内结核分枝杆菌标准株H37Rv杀灭对数值>4.00;杀灭标准株H37Ra需有效氯2 000 mg/L的消毒液。用有效氯1 000 mg/L的该消毒液作用2.5 min,对结核分枝杆菌多重耐药株a和c杀灭对数值均>4.00;杀灭多重耐药株d需要作用5 min,杀灭多重耐药株b需要作用10 min。从结核分枝杆菌标准菌株H37Rv和多重耐药菌株a与b中检出qacE△1-sulI基因,其他株未检出。所有试验菌株均未检出qacA/B基因。结论含氯消毒剂对结核杆菌标准株和多重耐药株均有良好杀菌作用。6株菌中qacE△1-sulI基因结果 3株阳性、3株阴性,而qacA/B基因均未检测到。
Objective To observe the killing effect of chlorine-containing disinfectant on drug-resistant Mycobacterium tuberculosis and to detect its resistance to disinfectant. Methods Suspension quantitative bactericidal test and gene amplification method were used to observe the effect of the chlorine disinfectant to kill Mycobacterium tuberculosis and to detect the gene. Results The logarithmic value of killing H37Rv of Mycobacterium tuberculosis in the suspension was> 4.00 with the chlorine disinfectant containing 1 000 mg / L of chlorine for 10 min. The standard strain H37Ra was required to kill 2 000 mg / L of available chlorine disinfectant. With the disinfectant solution of available chlorine at 1000 mg / L for 2.5 min, the killing logarithm values of multi-drug resistant strains a and c of Mycobacterium tuberculosis were all> 4.00; killing multi-drug resistant strains d for 5 min and killing Multi-drug resistant strains b need to act for 10 min. The qacE △ 1-sulI gene was detected from Mycobacterium tuberculosis standard strain H37Rv and the multi-drug resistant strains a and b, while the other strains were not detected. No qacA / B gene was detected in all the tested strains. Conclusion Chlorine-containing disinfectants have a good bactericidal effect against standard strains of Mycobacterium tuberculosis and multi-drug resistant strains. Of the 6 isolates, qacE △ 1-sulI gene was found to be 3 positive and 3 negative, while qacA / B was not detected.