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目的观察清热益气中药黄连人参对药对自发性2型糖尿病(T2DM)大鼠胰岛纤维化、脂联素(ADPN)水平及其受体(ADPNR)表达的影响。方法采用高热量高脂饲料喂养诱导GK大鼠,建立自发性T2DM大鼠模型。造模成功大鼠随机分为2组:模型组、黄连人参组,每组10只;另取雄性Wistar大鼠10只为正常对照组。黄连人参组以3.8 g/(kg·d)黄连人参水溶液(1.9 g生药/m L)灌胃,正常对照组和模型组灌服等量蒸馏水,持续给药4周。治疗前后进行OGTT试验,治疗后观察胰岛纤维化、ADPN及ADPNR在胰岛的表达情况。结果治疗前与正常对照组比较,模型组FPG与2h PG均升高(P<0.01)。治疗后与正常对照组比较,模型组大鼠体重、FPG与2h PG均升高(P<0.01),黄连人参组体重及2h PG较模型组降低(P<0.05)。与正常对照组比较,模型组胰岛内ADPNR1表达水平降低,胰岛内纤维结缔组织含量升高(P<0.01);与模型组比较,黄连人参组胰岛内ADPNR1表达水平升高(P<0.01),胰岛内纤维结缔组织含量降低(P<0.05)。3组间ADPN水平及ADPNR2表达比较,差异无统计学意义(P>0.05)。结论清热益气中药黄连人参可能通过上调ADPNR1表达,提高ADPN生物利用率,改善胰岛纤维化,促进胰岛损伤修复。
Objective To observe the effects of Qingre Yiqi Herbal Medicine, Huanglian ginseng on islet fibrosis, adiponectin (ADPN) and its receptor (ADPNR) expression in spontaneously type 2 diabetic rats. Methods GK rats were induced by high-calorie and high-fat diet to establish a spontaneous T2DM rat model. Rats were randomly divided into 2 groups: model group and Coptis ginseng group, with 10 rats in each group. Ten male Wistar rats were selected as normal control group. Coptis ginseng group gavage with 3.8 g / (kg · d) Coptis ginseng aqueous solution (1.9 g crude drug / m L), the normal control group and model group were fed with the same amount of distilled water, continuous administration for 4 weeks. OGTT test before and after treatment, after treatment observed islet fibrosis, ADPN and ADPNR in the islet expression. Results Compared with the normal control group before treatment, FPG and 2h PG in model group were both increased (P <0.01). Compared with the normal control group, body weight, FPG and 2h PG in the model group were significantly increased after treatment (P <0.01), and the body weight and 2h PG in the Coptis ginseng group were lower than those in the model group (P <0.05). Compared with the normal control group, the expression of ADPNR1 in islets and the content of fibrous connective tissue in islets were increased in the model group (P <0.01). Compared with the model group, the expression of ADPNR1 in the islets of the Coptis chinensis group increased (P <0.01) The content of fibrous connective tissue in islets decreased (P <0.05). ADPN levels and ADPNR2 expression between the three groups, the difference was not statistically significant (P> 0.05). Conclusion Radix Ginseng ginseng may improve ADPN bioavailability, improve pancreatic islet fibrosis and promote the repair of pancreatic islets by up-regulating the expression of ADPNR1.