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为了解安徽部分地区人群中嗜肺军团病杆菌Ⅰ型(LDB_1)血清抗体水平,我们于1985年10~12月对1518名正常人群的血清,用间接血凝法(PHA)进行了检测,结果报告如下。材料和方法一、血清:主要由本队抽取,部分由兄弟医院提供。其中体检人员血清1098份,非肺科的门诊病员血清420份。年龄为18~40岁,男1174名,女344名。军人(指在当地居驻一年以上)931名,当地居民587名。滁州、蚌埠、当涂三地区分别采血505、587、426份。二、抗原:用美国疾病控制中心(CDC)的LDB_1型菌株,接种于BCYE/legplp培养基35℃二氧化碳环境中培养5天,用灭菌蒸馏水洗下
In order to understand the serum level of LDB-1 antibody in the pneumophila Legionella pneumophila population in some areas of Anhui Province, we detected the serum of 1518 normal people from October to December 1985 by indirect hemagglutination (PHA) The report is as follows. Materials and methods First, serum: mainly drawn by the team, in part by the Brothers Hospital. Among them, 1098 were serum of medical staff and 420 were non-pulmonary outpatients. Aged 18 to 40 years old, male 1174, female 344. 931 military personnel (resident in the area for more than a year) and 587 local residents. Chuzhou, Bengbu, Dangtu three areas were blood 505,587,426 copies. Antigen: The LDB-1 strain of the CDC was inoculated on BCYE / legplp medium for 5 days at 35 ° C in a carbon dioxide atmosphere and washed with sterile distilled water