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随着生物工程及医药工业的蓬勃发展,氨基酸制剂的品种越来越多,因此氨基酸分析的重要性日益增加。目前已见报道的几种方法各有其优点,但不可避免的存在这样或那样的缺陷。以异硫氰酸苯酯(PITC)作为衍生化试剂不仅毒性大,且需要专门的衍生装置在无水状态下进行;另一种衍生剂4-(二甲氨基)偶氮苯-4’-硫酰氯(Dab-syl-CL)同氨基酸反应速度相对较慢,甚至需要升高温度,对于实现自动化。本文采取 OPA 和 FMOC 为衍生化试
With the rapid development of bioengineering and pharmaceutical industry, the variety of amino acid preparations is more and more, so the importance of amino acid analysis is increasing day by day. Several methods that have been reported so far each have their own advantages, but one or the other of these drawbacks inevitably exist. Phenylisothiocyanate (PITC) as a derivatizing agent is not only toxic, but also requires a special derivatization device in the anhydrous state; another derivative 4- (dimethylamino) azobenzene-4’- Dab-syl-CL reacts slowly with amino acids, requiring even elevated temperatures for automation. This article adopts OPA and FMOC as a derivative test