目的分析HIV抗体确证实验结果符合率,为提高实验室HIV抗体检测技术提供依据。方法按照《全国艾滋病检测技术规范》(2009版),对大连市2012—2013年初筛送检的1 119份阳性样品采用免疫印迹法(WB)进行确认。结果 (1)经确证实验,阳性率75.43%,不确定率15.46%,阴性率9.20%。(2)确证阳性的,男性占94.66%,女性占5.34%;未婚、已婚、离异分别占55.04%、24.08%、20.88%;29岁及以下占43.65%,30~39岁占24.08%,40~49岁占18.51%,50~59岁占9.37%,60岁及以上占4.39%;小学及以下、初中、高中、大专及大专以上分别占7.24%、24.44%、28.71%、39.62%。(3)WB带型种类分布,出现9条带的占40.69%,8条带占27.28%,7条占22.42%,6条占5.22%,5条占4.39%。(4)确证阳性的,env蛋白、pol蛋白、gag蛋白的平均阳性率分别为98.62%、94.74%、72.04%;不确定的,分别为46.32%、2.46%、36.14%。(5)WB带型年龄分布,3种env蛋白,5个年龄组差异均无统计学意义;3种gag蛋白差异均有统计学意义(P<0.05);p55,60岁及以上组明显低;p17,50~59岁组较高;3种pol蛋白,差异均有统计学意义(P<0.01),3种抗体的阳性率,50~59岁组均为100%,60岁及以上组均明显低。(6)不确定的样本完成追踪检测的96人,其中,转为阳性49人,占51.04%;转为阴性的23人,占23.96%。结论 HIV感染者男性多、未婚多、高学历多、低龄多,在大学要加强同性恋教育。不确定者的带型绝大多数不含抗pol基因编码的抗体。env蛋白与年龄关联小,gag蛋白与年龄有关联,pol蛋白与年龄关联明显。 Objective To analyze the coincidence rate of HIV antibody confirming experiment results and provide basis for improving HIV antibody testing technology in laboratory. Methods According to the “National AIDS Testing Technical Specification” (2009 Edition), 11 119 positive samples screened for screening in 2012-2013 in Dalian were confirmed by Western blotting (WB). Results (1) After confirmatory experiments, the positive rate was 75.43%, the uncertainty rate was 15.46%, the negative rate was 9.20%. (2) 94.66% were male and 5.34% were female; 55.04%, 24.08% and 20.88% were unmarried, married and divorced respectively; 43.65% were aged 29 and under, and they were 24.08% 40-49 years old accounted for 18.51%, 50-59 years old accounted for 9.37%, 60 years old and above accounted for 4.39%; primary and secondary, junior high school, high school, college and college accounted for 7.24%, 24.44%, 28.71%, 39.62% respectively. (3) Species distribution of WB bands, with 9 bands accounting for 40.69%, 8 bands accounting for 27.28%, 7 accounting for 22.42%, 6 accounting for 5.22% and 5 accounting for 4.39%. (4) The positive rates of positive env protein, pol protein and gag protein were 98.62%, 94.74% and 72.04% respectively, while the uncertainties were 46.32%, 2.46% and 36.14% respectively. (5) There was no significant difference in WB banding age, three env proteins and five age groups. The differences of three gag proteins were statistically significant (P <0.05); p55, 60 and older were significantly lower (P <0.01). The positive rates of the three antibodies in the age group of 50 to 59 years old were 100% and 60 years old and above, respectively Both were significantly lower. (6) 96 samples were tested by uncertain samples, of which 49 were positive, accounting for 51.04%; 23 were negative, accounting for 23.96%. Conclusions There are more men and more unmarried people with HIV infection, more educated people and younger children. Homosexuality education should be strengthened in universities. The majority of the unidentified bands are free of anti-pol gene-encoded antibodies. The relationship between env protein and age was small, gag protein was associated with age, and pol protein was significantly associated with age.