诱导性冷失活是 V型 ATPase的一种共同特性。用荧光光谱技术以水稻液泡膜 H+ -ATPase为研究对象 ,深入探讨了水稻幼苗冷失活现象的机制。研究表明 ,能引起较大程度冷失活现象的物质 ,往往先对膜脂有序性产生影响 ,通过对多种阳离子、阴离子、离子螯合剂导致冷失活能力和导致膜脂有序性变化能力间的比较 ,都能说明这一点。可以认为膜脂状态变化是冷失活机制发生的一个重要方面 ,也是植物早期寒害产生的原因之一。 Induced cold deactivation is a common feature of V-ATPase. Fluorescence spectroscopy was used to study the mechanism of cold inactivation of rice seedlings by using tonoplast membrane H + -ATPase as the research object. Studies have shown that substances that cause a greater degree of cold inactivation tend to have an effect on the orderliness of the membrane lipid first, by causing cold inactivation of a wide range of cationic, anionic, ion chelating agents and resulting in an orderly change in membrane lipid Comparison of ability, can illustrate this point. It can be considered that the change of membrane lipid status is an important aspect of cold inactivation mechanism and one of the causes of plant early chilling injury.