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目的:旨在完善乳癖消颗粒质量控制标准。方法:采用Waters C18柱(250 mm×4.6 mm,5μm)色谱柱,梯度洗脱(t:0→10 min→25 min→30 min→50 min→60 min→70 min→75 min→80 min,A:5%→15%→20%→45%→60%→80%→90%→5%→5%),柱温30℃,流速1.0 m L·min-1,载气压力40 psi,雾化温度42℃,飘逸管温度80℃。结果:芍药苷、三七皂苷R1、人参皂苷Rg1、连翘苷、丹皮酚、人参皂苷Rb1分别在13.66~204.90 ng(r=0.9994),23.88~358.20ng(r=0.9996),130.74~1961.10 ng(r=0.9996),8.54~128.10 ng(r=0.9997),9.78~146.70 ng(r=0.9994),92.16~1382.40 ng(r=0.9995)范围内呈良线性关系;芍药苷、三七皂苷R1、人参皂苷Rg1、连翘苷、丹皮酚、人参皂苷Rb1平均回收率分别为101.50%(RSD为2.10%),100.09%(RSD为2.77%),99.95%(RSD为1.81%),100.72%(RSD为2.68%),100.04%(RSD为1.96%),100.00%(RSD为2.01%)。结论:该方法灵敏、准确,分离效果较好,无干扰,可为乳癖消颗粒质量标准评价提供依据。
Objective: To improve the Rupixiao particles quality control standards. Methods: A Waters C18 column (250 mm × 4.6 mm, 5 μm) was used for gradient elution (t: 0 → 10 min → 25 min → 30 min → 50 min → 60 min → 70 min → 75 min → 80 min, A: 5% → 15% → 20% → 45% → 60% → 80% → 90% → 5% → 5%) at a flow rate of 1.0 m L · min- Atomization temperature 42 ℃, elegant tube temperature 80 ℃. Results: The concentrations of paeoniflorin, notoginsenoside R1, ginsenoside Rg1, forsythin, paeonol and ginsenoside Rb1 were 13.66 ~ 204.90 ng (r = 0.9994), 23.88 ~ 358.20 ng (r = 0.9996), 130.74 ~ 1961.10 (r = 0.9996), 8.54-128.10 ng (r = 0.9997), 9.78-146.70 ng (r = 0.9994) and 92.16-1382.40 ng (r = 0.9995) respectively. Paeoniflorin, notoginsenoside R1 , The mean recoveries of ginsenoside Rg1, forsythin, paeonol and ginsenoside Rb1 were 101.50% (RSD 2.10%), 100.09% (RSD 2.77%), 99.95% (RSD 1.81%) and 100.72% (RSD 2.68%), 100.04% (RSD 1.96%), 100.00% (RSD 2.01%). Conclusion: The method is sensitive and accurate, the separation effect is good, without interference, which can provide the basis for the evaluation of Rupixiao particle quality standard.