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为探讨痘苗病毒表达的Epslein Barr病毒 (EBV)核抗原 1、4(EBNA1、4)和潜伏膜蛋白 1、2 (LMP1、2 ) ,在不同人群的特异性T细胞引起的特异性T细胞杀伤 (CTL)中的作用 ,采集EBV阴性正常人、未经治疗的鼻咽癌 (NPC)病人和EBV IgA/VCA阳性者各 10人的周围血淋巴单核细胞 (PBMC) ,用EBV转化B淋巴细胞 ,建立类淋巴母细胞 (LCL)。用LCL刺激自体的T淋巴细胞作为效应细胞 ,以LCL感染重组痘苗病毒表达的EBNA1、4和LMP1、2为靶细胞 ,以51Cr释放法检测EBV特异性CTL所识别的靶抗原。结果表明 ,EBV LMP1、2可能既是EBV特异性T细胞的刺激抗原 ,又是其识别的靶抗原。将采集的 30例试验者的各 5株单克隆T细胞株分别检测HLA Ⅰ型(A、B、C) ,按照不同型别寻找相对应的EBNA1、4和LMP1、2的不同合成肽 ,应用酶免疫吸附斑点法 (Elispot)检测EBV特异性CD8+ 的CTL应答。结果显示 :10例正常人中 9人有特异的LMP2应答 ,4人有特异的EBNA4应答 ;10例未治疗的NPC病人中 3人有特异的LMP2 ,2人有特异的EBNA1,3人有特异的EBNA4应答 ;在 10例EBV-IgA/VCA阳性中 ,6人有特异的LMP2 ,5人有特异的EBNA4应答。所有的试验者均未发现LMP1的特异性应答
To investigate the specific T cell killing caused by vaccinia virus-specific Epstein-Barr virus (EBV) nuclear antigen 1,4 (EBNA1,4) and latent membrane protein 1,2 (LMP1,2) in different populations (CTL). Peripheral blood mononuclear cells (PBMCs) from 10 EBV-negative normal individuals, untreated NPC patients and EBV IgA / VCA positive individuals were collected. Cells, establishing lymphoid-like cells (LCLs). T lymphocytes stimulated with LCL as effector cells were infected with recombinant vaccinia virus LCL EBNA1,4 and LMP1,2 as target cells by 51Cr release method to detect EBV specific CTL recognized by the target antigen. The results showed that EBV LMP1,2 may be both EBV-specific T cell stimulating antigen, but also its recognition of the target antigen. HLA-A (A, B, C) was detected in 5 monoclonal T cell lines from 30 subjects tested, and different corresponding synthetic peptides of EBNA1,4 and LMP1,2 were screened according to different types. Enzyme-linked immunosorbent assay (Elispot) was used to detect CTL responses of EBV-specific CD8 +. The results showed that: 9 of 10 normal individuals had a specific LMP2 response and 4 had a specific EBNA4 response; 3 of 10 untreated NPC patients had a specific LMP2, 2 had a specific EBNA1 and 3 had a specific Of the EBNA4 responses; of the 10 EBV-IgA / VCA positives, 6 had a specific LMP2 and 5 had a specific EBNA4 response. No specific response to LMP1 was found in all subjects