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在Ｎ６ 附加秋水仙碱５０－０ 、１００－０ 、１５０－０、２００－０ 、２５０－０ 和３００－０ｍｇ／Ｌ 的培养基（ 对照为０－０ｍｇ／Ｌ） 上离体培养了Ｒｏｅｇｎｅｒｉａ ｃｉｌｉａｒｉｓ ｖａｒ．ｊａｐｏｎｅｎｓｉｓ ×Ｔｒｉｔｉｃｕｍ ａｅｓｔｉｖｕｍ ｃｖ．Ｊ－ １１、Ｒ．ｋａｍｏｊｉ×Ｈｏｒｄｅｕｍ ｖｕｌｇａｒｅ、Ｔｒｉｔｉｃｕｍ ａｅｓｔｉｖｕｍ ｃｖ．Ｃｈｉｎｅｓｅ Ｓｐｒｉｎｇ ×Ｐｓａｔｈｙｒｏｓｔａｃｈｙｓ ｈｕａｓｈａｎｉｃａ 和（ Ｔｒｉｔｉｃｕｍ ｄｕｒｕｍ ×Ｄａｓｙｐｙｒｕｍ ｖｉｌｌｏｓｕｍ） 双二倍体×Ｐ．ｈｕａｓｈａｎｉｃａ ４ 个杂交组合的小麦远缘杂种Ｆ１ 幼穗，结果发现，秋水仙碱对小麦远缘杂种Ｆ１ 幼穗脱分化具有明显的双重效应。一方面秋水仙碱具有促进小麦远缘杂种Ｆ１ 幼穗脱分化的正效应，表现为愈伤化时间明显早于对照，如杂交组合Ｒ．Ｃｉｌｉａｒｉｓｖａｒ．ｊａｐｏｎｅｎｓｉｓ×Ｔ．ａｅｓｔｉｖｕｍ ｃｖ．Ｊ－ １１ 和Ｔ．ａｅｓｔｉｖｕｍ ｃｖ．Ｃ．Ｓ×Ｐ．ｈｕａｓｈａｎｉｃａ于接种后一周内即有一半的外植体愈伤化，而对照始终没达到半愈期；同时，４ 个杂交组合的平均脱分化率显著高于对照，如秋水仙碱浓度为１?
Roegneria ciliaris were cultured in vitro on medium supplemented with colchicine N-6 at 50-0, 100-0, 150-0, 200-0, 250-0 and 300-0 mg / L (control 0-0 mg / L) var. japonensis × Triticum aestivum cv. J-11, R. kamoji × Hordeum vulgare, Triticum aestivum cv. Chinese Spring × Psathyrostachys huashanica and (Triticum durum × Dasypyrum villosum) diploid × P. huashanica. The results showed that colchicine had a distinct dual effect on the dedifferentiation of F1 spikelets in wheat hybrids. On the one hand, colchicine has the positive effect of promoting the dedifferentiation of F1 spikelets in wheat distant hybrids, which showed that the callus time was earlier than that of the control, such as hybrid R. Ciliarisvar. japonensis × T. aestivum cv. J-11 and T. aestivum cv. C. S × P. Huashanica callus harvested within one week after inoculation, while the control did not reach half-time; at the same time, the average dedifferentiation rate of four crosses was significantly higher than that of the control, such as colchicine concentration of 1?