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目的研究氟对大鼠肾脏细胞DNA损伤和细胞凋亡的影响。方法 48只SD大鼠随机分为4组(对照组、低氟组、中氟组和高氟组),每组12只,分别饮用含氟化钠为0、50、100和200 mg/L的去离子水,染毒120 d。动物处死后,用单细胞凝胶电泳技术(SCGE)检测氟化物对大鼠肾细胞DNA损伤,流式细胞术(FCM)检测细胞凋亡。结果彗星实验表明,各染氟组肾细胞拖尾率明显高于对照组(P<0.01)。染氟组大鼠肾细胞DNA迁移长度均高于对照组,高氟组与对照组比较,差异有统计学意义(P<0.01),中氟组与对照组比较,差异有统计学意义(P<0.05)。流式细胞术结果表明低氟组肾细胞凋亡率较对照组显著增高(P<0.05),中氟组、高氟组较对照组显著增高(P<0.01)。大鼠肾细胞DNA损伤与细胞凋亡之间存在明显的正相关关系(r=0.959,P<0.01)。结论氟可导致大鼠肾细胞DNA损伤,诱导肾细胞凋亡,氟诱导的大鼠肾细胞凋亡可能与氟所导致的DNA损伤有关。
Objective To study the effects of fluoride on DNA damage and apoptosis in rat kidney cells. Methods Forty eight Sprague-Dawley rats were randomly divided into 4 groups (control group, low fluoride group, medium fluoride group and high fluoride group), 12 rats in each group were dosed with 0, 50, 100 and 200 mg / L sodium fluoride Of deionized water, exposed to 120 d. After sacrificed, DNA damage of rat renal cells was detected by single cell gel electrophoresis (SCGE) and apoptosis was detected by flow cytometry (FCM). Results The comet assay showed that the tailing rate of renal cells in each fluoride group was significantly higher than that in the control group (P <0.01). The DNA migration length of kidney cells in the fluoridated group was higher than that in the control group, and there was significant difference between the high fluoride group and the control group (P <0.01), and there was significant difference between the middle fluoride group and the control group (P <0.05). The results of flow cytometry showed that the rate of renal cell apoptosis in low fluoride group was significantly higher than that in control group (P <0.05), and that in middle fluoride group and high fluoride group was significantly higher than that in control group (P <0.01). There was a significant positive correlation between DNA damage and apoptosis in rat renal cells (r = 0.959, P <0.01). Conclusion Fluoride can cause DNA damage in rat renal cells and induce renal cell apoptosis. Fluoride-induced renal cell apoptosis may be related to fluoride-induced DNA damage.