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目的探析肺癌细胞微生态环境中蛋白成分及其与人肺癌多药耐药细胞系SPC-A1/TAX耐药性之间的关系。方法通过基因芯片、蛋白质谱实验并借助S-P法以及MTS法来分析蛋白与耐药之间的关系。结果 SPC-A1/TAX与SPC-A1相比,除了对紫杉醇具有耐受性外,对其他抗肿瘤药物也有一定程度的耐药性。SPC-A1中,GST-π以及MRP的阳性率均为0,而SPC-A1/TAX中两者的阳性率均为100%,对其他抗肿瘤药物也有一定程度的耐药性,耐药倍数分别为6.34、2.42、13.51、2.65、9.80、7.00。结论多药耐药相关蛋白及谷胱甘肽-S-转移酶π与人肺癌多药耐药细胞系SPC-A1/TAX的耐药性密切相关,是导致其具有耐药性的重要机制。
Objective To investigate the relationship between protein components in microenvironment of lung cancer cells and drug resistance of multidrug-resistant human lung cancer cell line SPC-A1 / TAX. Methods The relationship between protein and drug resistance was analyzed by gene chip, protein profiling and S-P method and MTS method. Results Compared with SPC-A1, SPC-A1 / TAX showed some resistance to other antitumor drugs except paclitaxel. The positive rates of GST-π and MRP in SPC-A1 were all 0, while the positive rates of SPC-A1 / TAX in both of them were 100%, and there was a certain degree of resistance to other anti-tumor drugs. Respectively 6.34,2.42,13.51,2.65,9.80,7.00. Conclusions Multidrug resistance-associated protein and glutathione S-transferase π are closely related to the drug resistance of human lung cancer multidrug-resistant cell line SPC-A1 / TAX, which is an important mechanism leading to its drug resistance.