目的　为深入研究粘性放线菌Ⅱ型菌毛的粘附功能 ,对仅含Ⅱ型菌毛的T14V变异菌株 5 95 1细菌Ⅱ型菌毛的分子质量及亚单位组成进行初步分析。方法　采用免疫印迹实验对纯化的Ⅱ型菌毛进行分子质量检测。结果　显示分子质量为 31ku一条单一的色带 ,表明粘放菌 5 95 1菌毛是由相同的分子质量为 31ku的亚基组成。结论　根据文献报道及本实验结果推测 ,粘放菌 5 95 1菌毛分子质量为 12 4ku ,由 4个相同亚基组成。 Objective To further study the adhesion function of actinomyces viscosus type Ⅱ pili and analyze the molecular weight and subunit composition of the type Ⅱ pilose of T14V variant strain 5 951 containing only type Ⅱ pili. Methods Immunoblotting was used to detect the molecular weight of purified type Ⅱ pili. The results showed a single band of 31 ku in molecular mass, indicating that the 595 1 pili of the myxobacteria consisted of the same subunit of 31 ku. Conclusions According to the reports in the literature and the results of this experiment, it is speculated that the molecular weight of 5951 mycelia is 12 4ku, which consists of 4 identical subunits.