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本文用酸性α-醋酸萘酯酶(ANAE)反应标记淋巴细胞的细胞化学方法,对80例正常人外周血淋巴细胞的ANAE阳性率进行了观察,见到正常人外周血淋巴细胞的ANAE阳性率为74.1±7.3％,其中ANAE反应为“点状颗粒型”的淋巴细胞百分率为61.5±7.3％,“分散颗粒型”的淋巴细胞百分率为12.6±3.5％。同时我们对20例癌症患者(胃癌10例,乳腺癌5例,直肠癌5例)进行了观察,发现三组癌症患者外周血的淋巴细胞ANAE阳性率都显著地低于正常人(P<0.01),“点状颗粒型”淋巴细胞的百分率也显著地低于正常人(P<0.01),但是,“分散颗粒型”淋巴细胞的百分率和正常人比较没有差别。
In this paper, the cytotoxicity of ANAE-labeled lymphocytes was used to detect the positive rate of ANAE in 80 normal human peripheral blood lymphocytes. The ANAE positive rate of normal human peripheral blood lymphocytes Was 74.1 ± 7.3%. The percentage of lymphocytes with ANAE reaction as “dot-shaped granules” was 61.5 ± 7.3% and the percentage of “dispersed granules” with lymphocytes was 12.6 ± 3.5%. At the same time we observed 20 cases of cancer patients (10 cases of gastric cancer, 5 cases of breast cancer, 5 cases of rectal cancer) and found that the positive rate of ANAE of lymphocytes in peripheral blood of three cancer patients was significantly lower than that of normal people (P <0.01) ), And the percentage of “dot-shaped granular” lymphocytes was also significantly lower than that of normal subjects (P <0.01). However, the percentage of “scattered granular” lymphocytes did not differ from that of normal subjects.