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目的研究龙牙楤木多糖在大鼠体内的药代动力学。方法大鼠单次口服龙牙楤木多糖后,采用高效液相色谱与1-苯基-3-甲基-5-吡唑啉酮(PMP)柱前衍生方法测定大鼠血浆中的龙牙楤木多糖。色谱柱为Agilent Zorbax XDB-C18柱(5μm,150 mm×4.6 mm),流动相为0.1mol/L磷酸盐溶液(p H6.7):乙腈=83:17。结果血清样品中,龙牙楤木多糖的线性范围为5.0~100.0μg/ml(r=0.998 8);高、中、低3种不同浓度的日内RSD值分别为4.12%、6.28%、6.93%;日间RSD值分别为4.07%,6.50%,6.85%;相对回收率分别为88.0%、93.6%、95.07%。单剂量给药后,大鼠体内的药代动力学过程符合开放2室模型,主要药代动力学参数分别为Cmax:61.15μg/ml,tmax:94.57 min,AUC:20421.60 min/(μg·ml),t1/2ka:34.37,t1/2ke:149.17。结论高效液相色谱与PMP柱前衍生法测定大鼠血浆中的龙牙楤木多糖的方法专属性强,结果可靠。
Aim To study the pharmacokinetics of Aralia elata polysaccharide in rats. Methods After a single oral administration of Aralia elata polysaccharides, the aralia in rat plasma was determined by HPLC and 1-phenyl-3-methyl-5-pyrazolone (PMP) Alnus wood polysaccharides. The column was an Agilent Zorbax XDB-C18 column (5 μm, 150 mm × 4.6 mm) with a mobile phase of 0.1 mol / L phosphate solution (p H6.7): acetonitrile = 83:17. Results In the serum samples, the linear range of Aralia elata polysaccharides was 5.0-100.0μg / ml (r = 0.998 8). The daily RSD values of high, middle and low concentrations were 4.12%, 6.28% and 6.93% Day RSD values were 4.07%, 6.50% and 6.85%, respectively. The relative recoveries were 88.0%, 93.6% and 95.07% respectively. After single-dose administration, the pharmacokinetics in rats were in accordance with the open 2-compartment model. The main pharmacokinetic parameters were Cmax 61.15μg / ml, tmax 94.57min, AUC 20421.60 min / (μg · ml ), t1 / 2ka: 34.37, t1 / 2ke: 149.17. Conclusion The method of HPLC and PMP pre-column derivatization for the determination of Aralia elata polysaccharide in rat plasma is more specific and reliable.