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目的探讨ω-3多不饱和脂肪酸(PUFA)对失血性休克大鼠肠道细菌移位及存活时间的影响。方法 Wist-ar大鼠60只,随机分成假手术组(SHS)、失血性休克组(HS)及失血性休克+PUFA干预组(HS-F),每组各20只;观察各组大鼠存活时间、存活率及平均动脉压(MAP)动态变化,取肝、脾、肾及肠系膜淋巴结做细菌培养。结果 SHS、HS、HS-F组大鼠的存活时间分别为>180min、(113.0±25.9)min、(138.4±37.1)min,HS组与HS-F组大鼠的存活时间比较,差异有统计学意义(P<0.01);HS组及HS-F组较SHS组肝、脾、肾及肠系膜淋巴结细菌移位率明显升高(P<0.01);HS-F组较HS组肝、脾、肾及肠系膜淋巴结细菌移位率明显降低(P<0.01)。结论脂肪乳对HS大鼠肠黏膜屏障具有明显的保护作用,可有效降低HS后肠道细菌移位,并显著延长HS大鼠的存活时间。
Objective To investigate the effect of ω-3 polyunsaturated fatty acid (PUFA) on intestinal bacterial translocation and survival time in hemorrhagic shock rats. Methods Sixty Wist-ar rats were randomly divided into sham operation group (SHS), hemorrhagic shock group (HS) and hemorrhagic shock + PUFA intervention group (HS-F) Survival time, survival rate and mean arterial pressure (MAP) dynamic changes, to take liver, spleen, kidney and mesenteric lymph node bacterial culture. Results The survival time of rats in SHS, HS and HS-F groups were> 180min, (113.0 ± 25.9) min and (138.4 ± 37.1) min, respectively. The difference of survival time between HS group and HS- (P <0.01). The bacterial translocation rate of liver, spleen, kidney and mesentery lymph node in HS group and HS-F group was significantly higher than that in SHS group (P <0.01) The rate of bacterial translocation in renal and mesenteric lymph nodes was significantly lower (P <0.01). Conclusion Lipid milk has a significant protective effect on the intestinal mucosal barrier of HS rats, which can effectively reduce intestinal translocation of HS and significantly prolong the survival time of HS rats.