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目的:研究中药制剂海尔福口服液对汞染毒的治疗效果。方法:用小白鼠复制汞染毒动物模型,然后灌服海尔福口服液。将小鼠分成三组,每组20只。Ⅰ组是正常对照组;Ⅱ组是汞染毒不治疗组;Ⅲ组为汞染毒治疗组。Ⅱ、Ⅲ组先用HgCl2作腹腔注射,2周后Ⅲ组开始灌胃治疗,Ⅰ、Ⅱ组用等量的蒸馏水灌胃对照。实验共进行30天,测定小鼠实验前后的Hb。实验结束,测定血清尿素、肌酐、总蛋白、ALT及脑、肝MDA。结果:Ⅰ、Ⅱ、Ⅲ组治疗前Hb分别是(142.49±8.26)、(137.24±9.50)、(142.5±9.95)g/L(P>0.05);治疗后分别是(149.42±10.28)、(115.09±16.65)、(131.56±13.04)g/L(P<0.01或0.05);尿素分别为(5.60±0.39)、(10.99±1.03)、(9.27±1.59)mmol/L(P<0.01);肌酐分别为(124.77±14.75)、(149.70±12.16)、(148.06±16.16)nmol/L(P<0.01);血清总蛋白分别为(61.82±7.88)、(45.06±7.52)、(51.03±5.74)g/L(P<0.01);ALT分别为(16.97±7.88)、(27.25±7.52)、(26.0±9.08)U/L(P>0.05);脑MDA(每毫克蛋白)分别为(1.29±0.30)、(1.79±0.27)、(1.40±0.14)nmol/mg(P<0.01);肝MDA(每毫克蛋白)分别为(1.61±0.16)、(2.75±1.19)、(3.71±0.21)nmol/mg(P<0.01)。Ⅱ组死亡率明显高于Ⅰ组和Ⅲ组。结论:汞染毒明显损害造血、肾、肝组织,降低抗氧化能力,治疗后有明显的恢复。
Objective: To study the therapeutic effect of Chinese medicine preparation Haierfu oral solution on mercury exposure. METHODS: Rats were infected with a mercury-exposed animal model and then fed Haierfu oral solution. The mice were divided into three groups of 20 each. Group I was a normal control group; Group II was a mercury-untreated group; Group III was a mercury-exposed group. In groups II and III, HgCl2 was used as an intraperitoneal injection. After 2 weeks, group III began to be intragastrically administered. Groups I and II were treated with the same amount of distilled water. The experiment was conducted for a total of 30 days and the Hb before and after the mouse experiment was measured. At the end of the experiment, serum urea, creatinine, total protein, ALT, brain and liver MDA were measured. Results: The Hb before treatment in group I, II and III were (142.49±8.26), (137.24±9.50), (142.5±9.95) g/L (P>0.05); after treatment, they were (149.42±10.28), ( 115.09±16.65), (131.56±13.04)g/L (P<0.01 or 0.05); urea was (5.60±0.39), (10.99±1.03), (9.27±1.59) mmol/L (P<0.01); Creatinine was (124.77±14.75), (149.70±12.16), (148.06±16.16) nmol/L, respectively (P<0.01); serum total protein was (61.82±7.88), (45.06±7.52), (51.03±5.74), respectively. g/L (P<0.01); ALT was (16.97±7.88), (27.25±7.52), (26.0±9.08) U/L (P>0.05); brain MDA (per mg protein) was (1.29) (±0.30), (1.79±0.27), (1.40±0.14) nmol/mg (P<0.01); liver MDA (per mg protein) was (1.61±0.16), (2.75±1.19), (3.71±0.21) Nmol/mg (P<0.01). The mortality in Group II was significantly higher than Group I and III. Conclusion: Mercury exposure significantly damages hematopoietic, renal, and hepatic tissues, reduces antioxidant capacity, and restores significantly after treatment.