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目的:为了探讨低氧诱导因子-1α(Hypoxia inducible factor-1α,HIF-1α)在不同缺氧时间的肝癌细胞中的表达情况及其与血管生成和细胞凋亡的关系。方法:用氯化钴处理HepG2肝癌细胞模拟缺氧环境,用RT-PCR技术检测缺氧0,1,2,4,6,8h肝癌细胞中的HIF-1α,VEGF,bcl-2的表达,用westernblot方法检测caspase-3蛋白的表达情况。结果:细胞在氯化钴处理后4小时,HIF-1α,VEGF,bcl-2 mRNA达到高峰,随后下降;caspase-3蛋白的表达与前三者正好相反。结论:HIF-1α通过促进VEGF,bcl-2的表达,抑制caspase-3的表达,从而抑制肝癌细胞凋亡,且与缺氧程度有关。HIF-1α在肝癌的发生发展中发挥重要作用。
Objective: To investigate the expression of Hypoxia inducible factor-1α (HIF-1α) in hepatocellular carcinoma cells with different hypoxic time and its relationship with angiogenesis and apoptosis. Methods: HepG2 hepatocarcinoma cells were treated with cobalt chloride to simulate hypoxia environment. The expression of HIF-1α, VEGF and bcl-2 in hypoxic cells were detected by RT-PCR at 0, 1, 2, Western blot was used to detect the expression of caspase-3 protein. Results: The mRNA level of HIF-1α, VEGF and bcl-2 peaked at 4h after treatment with cobalt chloride, and then decreased. The expression of caspase-3 protein was opposite to the former three. Conclusion: HIF-1α can inhibit the apoptosis of hepatocellular carcinoma cells by promoting the expression of VEGF and bcl-2 and inhibiting the expression of caspase-3, which is related to the degree of hypoxia. HIF-1α plays an important role in the occurrence and development of liver cancer.