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目的探讨基质金属蛋白酶-1(MMP-1)、基质金属蛋白酶组织抑制剂-1(TIMP-1)及转化生长因子-β1(TGF-β1)在风湿性心脏病(RHD)瓣膜组织中的表达及意义。方法采用Western blot及RT-PCR法检测RHD瓣膜组织(实验组,n=30)及无RHD瓣膜组织(对照组,n=15)中MMP-1、TIMP-1及TGF-β1蛋白与mRNA的表达,碱水解法检测胶原代谢情况,Masson法进行胶原纤维染色并检测胶原容积分数(CVF)。分析MMP-1、TIMP-1及TGF-β1与CVF的相关性。结果 MMP-1及TGF-β1蛋白和mRNA在实验组中的表达高于对照组,而TIMP-1蛋白和mRNA在实验组中的表达低于对照组,差异有统计学意义(P<0.05)。实验组胶原含量及CVF均高于对照组(P<0.05)。相关性分析显示,MMP-1与TGF-β1呈正相关,TIMP-1与TGF-β1呈负相关,MMP-1及TGF-β1与CVF呈正相关,TIMP-1与CVF呈负相关。结论 MMP-1、TGF-β1和TIMP-1参与了RHD瓣膜病变的纤维化进展。
Objective To investigate the expression of matrix metalloproteinase-1 (MMP-1), matrix metalloproteinase-1 (TIMP-1) and transforming growth factor-β1 (TGF-β1) in rheumatic heart disease (RHD) And meaning. Methods Western blot and RT-PCR were used to detect the expressions of MMP-1, TIMP-1 and TGF-β1 in RHD valve tissue (experimental group, n = 30) and non-RHD valve tissues (control group, n = 15) Expression and alkaline hydrolysis were used to detect collagen metabolism. Collagen staining and collagen volume fraction (CVF) were detected by Masson’s method. The correlation between MMP-1, TIMP-1, TGF-β1 and CVF was analyzed. Results The expressions of MMP-1 and TGF-β1 protein and mRNA in the experimental group were higher than those in the control group, while the expressions of TIMP-1 protein and mRNA in the experimental group were lower than those in the control group (P <0.05) . Collagen content and CVF in experimental group were higher than those in control group (P <0.05). Correlation analysis showed that there was a positive correlation between MMP-1 and TGF-β1, negative correlation between TIMP-1 and TGF-β1, positive correlation between MMP-1 and TGF-β1 and CVF, and negative correlation between TIMP-1 and CVF. Conclusion MMP-1, TGF-β1 and TIMP-1 are involved in fibrosis of RHD valvular lesions.