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目的了解长寿老人的人类白细胞抗原DQA1、DQB1(HLADQA1、HLADQB1)基因多态性的特点,探讨HLA与长寿的相关性。方法应用聚合酶链式反应序列特异引物(PCRSSP)对东兰县150名90岁以上壮族长寿老人(90~105岁)和143名当地健康、无血缘关系、随机抽样的壮族成年人(20~76岁)进行HLADQA1和HLADQB1两座位的基因分型并进行相应等位基因频率的比较。结果①长寿组等位基因HLADQA10102的频率明显高于对照组(分别为4133%和2832%,P<005);②长寿组HLADQA10101及HLADQB10301的频率分别为367%和900%,明显低于对照组的1364%和2168%(P均<001)。③其余各相应等位基因频率之间的比较无显著差异(P值均>005)。相关分析显示,HLADQA10102与长寿呈正相关,而DQA10101及DQB10301呈负相关(OR分别为13083、02410及03296;P分别<005、001及001)。结论在东兰县壮族人群中,带有HLADQA10102等位基因的个体可能有利于长寿,而HLADQA10101和DQB10301则可能是长寿的不利因子。
Objective To understand the characteristics of human leukocyte antigen DQA1, DQB1 (HLADQA1, HLADQB1) gene in longevity elderly and to explore the relationship between HLA and longevity. Methods A total of 150 Zhuang long-term elderly (90-105 years old) over 90 years old and 143 healthy, unrelated and randomly sampled Zhuang adults (20-20 years old) from Donglan County were enrolled in this study. 76 years old) were genotyped for the two loci of HLADQA1 and HLADQB1 and the corresponding allele frequencies were compared. Results ① The frequency of HLADQA10102 in the longevity group was significantly higher than that in the control group (4133% and 2832% respectively, P <005). ② The frequencies of HLADQA10101 and HLADQB10301 in longevity group were 367% and 900% Of 1364% and 2168% (all P <001). ③ The rest of the corresponding allele frequencies between the no significant difference (P value> 005). Correlation analysis showed that HLADQA10102 was positively correlated with longevity, while DQA10101 and DQB10301 were negatively correlated (OR = 13083, 02410 and 03296 respectively; P <005, 001 and 001, respectively). Conclusion In Zhuang nationality in Donglan County, individuals with HLADQA10102 allele may be beneficial to longevity, while HLADQA10101 and DQB10301 may be unfavorable factors for longevity.