论文部分内容阅读
目的：探讨维生素A抑制Shope肿瘤细胞生长的机理。方法：电镜观察Shope肿瘤细胞凋亡的形态学改变：琼脂糖凝胶电泳进行DNA片段化；流式细胞仪检测凋亡细胞的百分比。结果：电镜下可VA诱导的Shope肿瘤细胞变小，细胞浆固缩，染色质聚集并靠近核膜，在1．5％琼脂糖凝胶电泳中，Shope肿瘤细胞DNA呈现明显的阶梯状图谱。流式细胞仪检测经10－5MV作用24h、48h、72h后，凋亡细胞百分比分别为：17．1％、22．4％、32．4％。结论：以上结果表明，VA对Shope肿瘤细胞有明显的抑制作用。
Objective: To investigate the mechanism of vitamin A inhibiting the growth of Shope’s tumor cells. Methods: The morphological changes of apoptosis in Shope’s tumor cells were observed by electron microscopy. DNA fragmentation was performed by agarose gel electrophoresis; the percentage of apoptotic cells was detected by flow cytometry. RESULTS: Under the electron microscope, the VA-induced Shope tumor cells became smaller, the cytoplasm was pyknosis, and the chromatin aggregated near the nuclear membrane. Shopin tumor cell DNA showed a ladder-like pattern in a 1.5% agarose gel electrophoresis. The percentage of apoptotic cells after treatment with 10-5MV for 24h, 48h, and 72h by flow cytometry was 17.1%, 22.4%, and 32.4%, respectively. Conclusion: The above results show that VA has a significant inhibitory effect on Shope’s tumor cells.