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目的探讨乙氧氟草醚原药的致突变作用。方法将昆明种小鼠60只,雌雄各半,随机分为6组,每组10只。实验组分别以625、1 250、2 500、5 000 mg/kg乙氧氟草醚原药灌胃染毒,连续2 d,每天1次；阳性对照给予环磷酰胺(50 mg/kg)；阴性对照给予植物油灌胃。将雄性昆明种小鼠25只,随机分为5组,每组5只。实验组分别以250、500、1000 mg/kg乙氧氟草醚原药灌胃染毒,连续5 d,每天1次；阳性对照给予环磷酰胺(50 mg/kg)；阴性对照给予植物油。分别取胸骨、睾丸组织,常规制片,镜下观察骨髓多染红细胞微核率及睾丸精母细胞畸变细胞率。以乙氧氟草醚分别为500、1 000、2 000、5 000μg/皿,进行鼠伤寒沙门菌回复突变(Ames)试验。结果各剂量实验组小鼠骨髓多染红细胞微核率、睾丸精母细胞染色体畸变细胞率与阴性对照组比较,差异均无统计学意义(P＞0．05)；Ames实验显示各菌株各剂量组的回变菌落数均未超过自发回变菌落数的2倍。结论在该试验条件下,未发现乙氧氟草醚原药的致突变性。
Objective To investigate the mutagenic effect of oxyfluorfen original drug. Methods Kunming mice 60, male and female, were randomly divided into 6 groups of 10. The experimental group were orally administered with 625, 1 250, 2 500 and 5 000 mg / kg of oxyfluorfen respectively, twice a day for 2 days. The positive control group was given cyclophosphamide (50 mg / kg) Negative control given vegetable oil gavage. Twenty-five male Kunming mice were randomly divided into five groups of five. The experimental group were administered orally with 250, 500, 1000 mg / kg oxyfluorfen respectively, once a day for 5 consecutive days. The positive control was given cyclophosphamide (50 mg / kg). The negative control was given to the vegetable oil. The sternum, testis tissue, routine preparation were made respectively. The micronucleus rate of erythrocytes in micronucleus and the rate of aberrant cells of testicular spermatocytes were observed microscopically. Salmonella typhimurium reversion (Ames) test was conducted with oxyfluorfen at 500, 1 000, 2 000 and 5 000 μg / dish, respectively. Results The bone marrow erythrocyte micronuclei rate and the rate of testicular spermatocyte chromosome aberration in test group were not significantly different from those in negative control group (P> 0.05). Ames experiment showed that the dose of each strain The number of back-changing colonies in the group did not exceed 2 times the number of spontaneous back-changing colonies. Conclusions Under this test condition, no mutagenicity of the original oxyfluorfen drug was found.