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目的研究皮肤组织中细胞外基质(ECM)成分的糖基化对成纤维细胞(FB)黏附增殖能力的影响。方法对糖尿病患者皮肤组织中晚期糖基化终末产物(AGEs)的表达进行免疫组化测定;体外将FB的ECM糖基化处理,观察FB细胞的形态变化和糖基化基质对其黏附、增殖能力的影响,以非糖尿病皮肤组织作为对照。结果糖尿病患者皮肤组织中FB的细胞浆和ECM中AGEs的阳性表达明显多于非糖尿病皮肤;体外糖基化基质上接种的FB较小,胞体突起既少又短,黏附的细胞明显少于对照组(P<0.01);接种1 h,黏附细胞增多,逐渐与对照组接近,但仍低于对照组。结论糖尿病患者皮肤组织中的ECM被糖基化,产生的AGEs可抑制FB的黏附、增殖,可能是糖尿病创面愈合延迟的重要机制之一。
Objective To study the effect of glycosylation of extracellular matrix (ECM) in skin tissue on the proliferation and adhesion of fibroblasts (FBs). Methods The expression of advanced glycation end products (AGEs) in the skin of diabetic patients was determined by immunohistochemistry. The ECs of FB were glycosylated in vitro and the morphological changes of FB cells and the adhesion of glycosylation matrix were observed. Proliferative capacity of non-diabetic skin tissue as a control. Results The positive expression of AGEs in the cytoplasm of FB and ECM in diabetic patients was significantly higher than that in non-diabetic skin. The FBs inoculated on in vitro glycosylated matrices were smaller and the soma of cytoplasm were smaller and shorter, with less adherent cells Group (P <0.01). At 1 hour after inoculation, the number of adherent cells increased, gradually approaching to the control group, but still lower than that of the control group. Conclusion ECM in the skin of diabetic patients is glycosylated. The produced AGEs can inhibit the adhesion and proliferation of FB, which may be one of the important mechanisms of delayed wound healing in diabetic patients.