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实验将新生大鼠脑顶盖或坐骨神经段分别移植到成年大鼠受损的视神经断端或眼玻璃体内,动物存活4周后取视网膜,切片,Nissl染色,显微镜观察,并用计算机图像分析仪对部分视网膜节细胞胞体截面积进行测量和统计学处理.结果可见,异体移植其中一种移植物后,均可使部分受损的视网膜节细胞暂不演变,仍存活,胞体呈增大的形态学变化.当移植物为坐骨神经时,如移植到眼玻璃体内可使视网膜节细胞胞体明显增大,截面积可增至41.8±34.3μm~2,其中巨大视网膜节细胞(截面积50μm~2以上)占23.8%;如移植到视神经断端效果则次之,经统计学处理二者有显著性差异(P<0.01).当两种移植物分别移至视神经断端时,视网膜节细胞胞体截面积增大幅度则相似,分别为24.4±10.2μm~2和23.3±10.1μm~2,巨大视网膜节细胞构成比仅占1.5%和1.0%.
In the experiment, the newborn rat brains or the sciatic nerve were transplanted into the injured optic nerve or the vitreous body of the adult rats respectively. The animals survived for 4 weeks and then the retina was sectioned, Nissl staining and microscopic observation were performed. Part of the retinal ganglion cell body cross-sectional area measurement and statistical treatment.Results, we can see that after allograft transplantation, part of the damaged retinal ganglion cells can not evolve, still survive, the cell body increased morphology Changes.When the graft is sciatic nerve, such as transplanted into the vitreous of the eye can make retinal ganglion cell body significantly increased, the cross-sectional area can be increased to 41.8 ± 34.3μm2, in which the giant retinal ganglion cells (cross-sectional area of 50μm ~ 2 above) Accounting for 23.8%; if the transplanted to the optic nerve end effect is the second, statistically significant difference between the two (P <0.01) .When the two grafts were moved to the optic nerve ends, retinal ganglion cell body cross-sectional area The amplitudes were similar with 24.4 ± 10.2μm ~ 2 and 23.3 ± 10.1μm ~ 2, respectively. The proportion of giant retinal ganglion cells was only 1.5% and 1.0% respectively.