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本文以棉铃虫 3龄幼虫为试虫材料对增效醚 (PBO)的毒理学进行了研究。结果表明 ,PBO对棉铃虫(H elicoverp a armigera) 3龄幼虫的 L D50 为 5.7532 μg/ larva;在活体抑制实验中 ,用 4 μg/ larva的剂量点滴处理1h后 ,幼虫体内乙酰胆碱酯酶 ACh E的活性被抑制 4 8.31% ,羧酸酯酶 Carb E被抑制 4 4 .97% ,多功能氧化酶MFO被抑制 6 0 .82 % ;在离体抑制试验中 ,PBO对 ACh E、Carb E和 MFO的抑制中率 (I50 )分别为 3.35× 10 -7mol/ L、4 .4 6× 10 -7mol/ L和 1.4 2× 10 -7mol/ L ;PBO对酯酶的抑制为不可逆抑制。因此 ,PBO的增效机理在抑制代谢酶系的水平上并不是专一的 ;对于棉铃虫而言 ,PBO具有明显的杀虫活性 ,用其进行增效实验判断MFO是否参与抗性时应注意 PBO的这一特点 ;同时文章还证明了磷酸三苯酯 (TPP)和顺丁烯二酸二乙酯(DEM)抑制作用的专一性
In this paper, the third instar larvae of Helicoverpa armigera were used to study the toxicology of PBO. The results showed that the L D50 of PBO to 3rd instar larvae of H. armigera was 5.7532 μg / larva. In the in vivo inhibition experiment, after treated with 4 μg / larva for 1 h, the acetylcholinesterase ACh E Activity was inhibited by 4 8.31%, carboxylesterase Carb E was inhibited by 4 4 .97% and multifunctional oxidase MFO was inhibited by 60.82%. In vitro inhibition test, PBO showed no significant effect on ACh E, Carb E and The MFO inhibition rates (I50) were 3.35 × 10 -7 mol / L, 4.46 × 10 -7 mol / L and 1.4 2 × 10 -7 mol / L, respectively. The inhibition of esterase by PBO was irreversible. Therefore, PBO synergistic mechanism in inhibiting the level of metabolic enzymes is not specific; for the cotton bollworm, PBO has significant insecticidal activity, with its synergistic experiment to determine whether the participation of MFO resistance should pay attention to PBO this feature; the article also demonstrated that the specificity of the inhibition of triphenyl phosphate (TPP) and maleic acid diethyl ester (DEM)