匐柄霉对大丽轮枝菌生长及微菌核形成的影响

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在离体条件下及棉花植株内测试了匐柄霉对大丽轮枝菌的抑制作用。结果表明,在离体条件下,匐柄霉菌的菌丝体或其培养滤液,对大丽轮枝菌的生长和微菌核形成均有影响。将形态发生变异,不能形成微菌核的大丽轮枝菌菌株转移至PDA培养基后,其微菌核形成能力不再恢复。匐柄霉培养滤液的抑菌物质受热不稳定,100℃处理10分钟后活性丧失。抑菌物质经硫酸铵、乙醇沉淀初步测定,属非蛋白次生代谢产物。匐柄霉经接种可进入棉株体内,定殖率为75%~100%,分布于棉苗根、茎的维管束组织中。与轮枝菌先后或同时混合接种,匐柄霉可减轻棉花黄萎病的症状,以棉苗栽种前用匐柄霉菌分生孢子悬浮液沾根处理效果最明显。 Inhibition of Verticillium dahliae by Tricholoma pallidum was tested in vitro and in cotton plants. The results showed that in vitro, the mycelia of Castanopsis oryzae or its culture filtrate had an effect on the growth and micro-sclerotia of Verticillium dahliae. When the morphological changes occurred, the strains of Verticillium dahliae that could not form the microscopic nucleus transferred to the PDA medium and their ability to form the bacterial core no longer recovered. Curie fungus culture filtrate of the antimicrobial substances unstable heat, 100 ℃ for 10 minutes after the loss of activity. Bacteriostatic substances by ammonium sulfate, ethanol precipitation preliminary determination, is a non-protein secondary metabolites. Castanopsis kawakamii can be inoculated into the cotton plant in vivo, colonization rate of 75% to 100%, distributed in cotton seedling root, stem vascular tissue. Verticillium fungi and mixed with inoculation at the same time, Scutellaria rolfsii can reduce the symptoms of cotton Verticillium wilt, before cotton seedlings planted with the fungi conidia suspension conidia treatment effect is most obvious.
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