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目的:探讨血管紧张素Ⅱ(AngⅡ)受体(AT1,AT2)拮抗剂对梗死心肌组织中钙蛋白酶(calpain)系统的作用。方法:结扎大鼠左冠状动脉建立心肌梗死(MI)模型,将成功造模的96只大鼠分为4组,每组24只。各组术后1、3、7、14d分别检测左心室游离壁(LVFW)、室间隔(IS)、右室壁(RV)的calpainⅠ、Ⅱ及钙蛋白酶抑制蛋白(calpastatin)表达。结果:术后14d,手术组IS的calpainⅠ蛋白表达与LVFW的calpainⅡ蛋白表达均明显高于假手术组、手术加缬沙坦组及手术加PD123319组(均P<0·01);各组LVFW、RV的calpainⅠ蛋白表达,IS、RV的calpainⅡ蛋白表达以及IS、LVFW、RV的calpastatin蛋白表达差异均无统计学意义。结论:calpainⅠ参与MI的晚期重塑,calpainⅡ参与早期缺血心肌组织的损伤作用。MI病理过程中,calpainⅠ和Ⅱ的上调通过AT1起作用,AT1受体拮抗剂的心脏保护作用与其抑制了calpain系统有关。
Objective: To investigate the effect of angiotensin Ⅱ receptor (AT1, AT2) antagonist on calpain system in infarcted myocardium. Methods: Myocardial infarction (MI) model was established by ligation of the left coronary artery in rats. 96 successful rats were divided into 4 groups with 24 rats in each group. The left ventricular free wall (LVFW), interventricular septum (IS), and the expression of calpain I, II and calpastatin in RV were detected at 1, 3, 7 and 14 days after operation. Results: The expressions of calpain Ⅰ protein and calpain Ⅱ protein in IS group were significantly higher than those in sham operation group, valsartan group and PD123319 group (all P <0.01) after operation. The LVFW , RV calpain Ⅰ protein expression, IS, RV calpain Ⅱ protein expression and IS, LVFW, RV calpastatin protein expression differences were not statistically significant. Conclusion: Calpain Ⅰ is involved in the remodeling of MI and calpain Ⅱ is involved in the injury of early ischemic myocardium. During the pathological process of MI, the up-regulation of calpain I and II through AT1 plays a role. The cardioprotection of AT1 receptor antagonist is related to its inhibition of calpain system.