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抗精子抗体 (AsAb)的测定是诊断免疫性不育的重要指标之一 ,AsAb的类型和含量对精子的功能有较大的影响。本文就AsAb定量测定进行了研究。TIg ,IgA和IgG的测定上限可达 5 0 0U/ml,回收率在 96 4 %～10 3 %之间 ,批内重复性 <7 0 %。参考值范围为TIg <74 3U/ml,IgA <5 5 2U/ml和IgG <61U/ml。经在临床的初步应用表明 ,在不育患者中TIg超过参考值上限的比例男性 2 3 7% ,女性 3 1 1% ,但无统计学差异 ;血清中TIg与TIG具有较好的相关性 ,而与IgA有一定的相关性 ,可同时测定TIg和IgA对不育患者进行诊断。
Determination of anti-sperm antibodies (AsAb) is one of the important indicators of diagnosis of immune infertility, AsAb type and content of sperm function has a greater impact. In this paper, AsAb quantitative determination was studied. The upper limit of determination of TIg, IgA and IgG is up to 500U / ml, the recoveries are between 96 4% -103%, and the intra-assay repeatability is <70%. The reference range is TIg <74 3 U / ml, IgA <5 5 2 U / ml and IgG <61 U / ml. The preliminary clinical application showed that TIg exceeded the upper limit of reference value in infertile men by 23.7% and 31.1% of women, but there was no significant difference. There was a good correlation between TIg and TIG in serum, And with a certain degree of correlation with IgA, TIg and IgA can be simultaneously determined for infertility patients.