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目的探讨骨髓间充质干细胞(MSCs)同种移植治疗急性心肌梗死的可行性。方法50只Wistar大鼠随机分为两组,对照组和移植组各25只。应用液氮冷冻法制作心肌梗死模型。移植组梗死部位注入经5-氮胞苷(5-aza)诱导和溴氮胞苷标记的大鼠MSCs,对照组梗死部位注入等容量DMEM液。分别于术前1天、术后1周和术后4周行超声心动图检查,以收缩期峰值速度(Vs)、左室舒张末容积(LVEDV)和左室射血分数(LVEF)为指标评价心功能。移植组于术后4周处死,移植心肌行溴氮胞苷免疫组化染色。结果对照组(n=13)和移植组(n=15)相比,术前及术后1周的心功能差异均无统计学意义(P>0.05),术后4周差异有统计学意义(P<0.05),术后4周移植组心功能明显优于对照组。移植部位免疫组化可见溴氮胞苷染色阳性细胞。结论经5-aza诱导的MSCs同种移植入大鼠急性心肌梗死模型的受损心肌后能存活并改善宿主的心功能。
Objective To investigate the feasibility of bone marrow mesenchymal stem cells (MSCs) allografting in the treatment of acute myocardial infarction. Methods Fifty Wistar rats were randomly divided into two groups, 25 in control group and transplantation group. Myocardial infarction model was made by liquid nitrogen freezing method. Transplantation group were infused with 5-azacytidine (5-aza) -induced MSCs and brominated azacytidine-labeled MSCs into the infarcted area of the transplantation group. The control group was infused with equal volume of DMEM solution. Echocardiography was performed at 1 day before surgery, 1 week after surgery and 4 weeks after surgery. Peak systolic velocity (Vs), left ventricular end-diastolic volume (LVEDV) and left ventricular ejection fraction (LVEF) Evaluation of heart function. The transplantation group was sacrificed 4 weeks after the operation, and the hearts were transplanted for immunohistochemical staining with cytarabine. Results There was no significant difference in cardiac function between the control group (n = 13) and the transplantation group (n = 15) before and after 1 week (P> 0.05), and the difference was statistically significant at 4 weeks after operation (P <0.05). At 4 weeks after transplantation, the cardiac function in the transplantation group was significantly better than that in the control group. Transplantation of immunohistochemical staining of BDNF positive cells. Conclusion MSCs induced by 5-aza can be allografted into injured myocardium of acute myocardial infarction model in rats and improve cardiac function.