目的:建立复方黄根颗粒(无糖型)中有效成分三七皂苷R1、人参皂苷Rg1和人参皂苷Rb1的含量测定方法。方法:色谱柱为Agilent ZORBAX SB-C18(250 mm×4.6 mm,5μm),以乙腈-水梯度洗脱,流速为1.0 ml·min-1,检测波长为203 nm,柱温25℃,进样量为10μl。结果:三七皂苷R1、人参皂苷Rg1和人参皂苷Rb1的线性范围分别为1.6~10.0μg·ml-1(r=0.999 6)、6.3~39.3μg·ml-1(r=0.999 8)和6.3~39.7μg·ml-1(r=0.999 7),平均加样回收率分别为98.81%(RSD=1.20%)、99.93%(RSD=0.93%)和99.22%(RSD=0.87%)(n=6)。结论:本方法操作简便、重复性好,可以更有效地控制该制剂的质量。 Objective: To establish a method for the determination of notoginsenoside R1, ginsenoside Rg1 and ginsenoside Rb1 in Fufang Huanggen granules (no-sugar type). METHODS: The column was Agilent ZORBAX SB-C18 (250 mm × 4.6 mm, 5 μm), eluted with a gradient of acetonitrile-water at a flow rate of 1.0 ml · min-1 at a detection wavelength of 203 nm with a column temperature of 25 ° C The amount is 10 μl. Results: The linear ranges of notoginsenoside R1, ginsenoside Rg1 and ginsenoside Rb1 were 1.6 ~ 10.0μg · ml-1 (r = 0.999 6), 6.3 ~ 39.3μg · ml-1 (r = 0.999 8) and 6.3 (RSD = 0.93%) and 99.22% (RSD = 0.87%) (n = 6). Conclusion: The method is simple, reproducible and can control the quality of the preparation more effectively.