论文部分内容阅读
诱导广谱有效的中和抗体被认为是预防性疫苗成功的关键,HIV中和抗体检测方法的建立和标准化为HIV疫苗评价和设计提供指导,也为不同HIV疫苗的比较提供了平台。本文从方法的建立、假病毒株的选择、评价标准的制定、评价策略的确定、该方法在HIV其它研究领域的应用等方面,对目前应用最广泛的以假病毒为基础的中和抗体评价方法进行了综述。
The induction of a broad spectrum of effective neutralizing antibodies is considered as the key to the success of preventive vaccines. The establishment and standardization of HIV neutralizing antibody detection methods provide guidance for the evaluation and design of HIV vaccines and provide a platform for comparison of different HIV vaccines. In this paper, the establishment of the method, the selection of pseudoviruses, the formulation of evaluation criteria and the determination of evaluation strategies are reviewed. The method is applied to evaluate the neutralizing antibodies based on pseudoviruses Methods are reviewed.