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目的:研究粘附分子(MUC4)、核转录因子(NF-κB)p65和血管内皮生长因子(VEGF)在卵巢上皮性癌组织中的表达及意义。旨在为卵巢上皮性癌的早期诊断及预后的判断寻找标志,并为卵巢上皮性癌的发病和转移机制提供理论依据。方法:应用免疫组化SP法,检测MUC4、p65和VEGF在10例正常卵巢组织、20例卵巢良性上皮性肿瘤和60例卵巢上皮性癌组织中的表达,并分析它们的相关性。结果:卵巢上皮性癌组织中,MUC4阳性表达率显著高于正常卵巢组织及卵巢良性肿瘤组织,P<0.05。MUC4的表达与病理分级有关,χ2=14.332,P=0.008;与临床分期、CA125值和有无淋巴结转移等无关,P>0.05。p65及VEGF的表达与淋巴结转移、临床分期、病理分级和CA125值有关,P<0.05;VEGF与腹水形成有关,χ2=10.651,P=0.032。在Ⅰ～Ⅱ期卵巢上皮性癌中,MUC4的阳性表达率显著高于p65和VEGF,P<0.05。p65和VEGF、MUC4的表达呈正相关(r=0.209,P=0.048;r=0.279,P=0.008)。结论:MUC4是上皮性卵巢癌早期诊断的候选指标,VEGF与预后有关;p65对MUC4和VEGF有调控作用。
Objective: To investigate the expression and significance of MUC4, NF-κB p65 and VEGF in epithelial ovarian cancer. The purpose of this study is to find a marker for the early diagnosis and prognosis of ovarian epithelial carcinoma and to provide a theoretical basis for the pathogenesis and metastasis of epithelial ovarian cancer. Methods: The expressions of MUC4, p65 and VEGF in 10 cases of normal ovarian tissue, 20 cases of ovarian benign epithelial tumor and 60 cases of ovarian epithelial carcinoma were detected by immunohistochemical SP method and analyzed their correlation. Results: The positive rate of MUC4 in ovarian epithelial carcinoma was significantly higher than that in normal ovarian tissue and benign ovarian tumor (P <0.05). MUC4 expression and pathological grade, χ2 = 14.332, P = 0.008; and clinical stage, CA125 value, with or without lymph node metastasis, etc., P> 0.05. The expression of p65 and VEGF was correlated with lymph node metastasis, clinical stage, pathological grade and CA125 value, P <0.05. VEGF was associated with the formation of ascites, χ2 = 10.651, P = 0.032. In stage Ⅰ ~ Ⅱ ovarian epithelial carcinoma, the positive expression rate of MUC4 was significantly higher than that of p65 and VEGF (P <0.05). The expression of p65 and VEGF, MUC4 were positively correlated (r = 0.209, P = 0.048; r = 0.279, P = 0.008). Conclusions: MUC4 is a candidate indicator for the early diagnosis of epithelial ovarian cancer. VEGF is associated with prognosis. P65 may play a regulatory role in MUC4 and VEGF.