Activities of selected soil enzymes (invertase, acid phosphatase, proteinase, catalase, peroxidase and polyphenoloxidase) were determined under different spruce forests with restoration histories of 5, 13, 18, 23, 27 years and an old growth forest over 400 years old in the eastern Qinghai-Tibetan Plateau, China, and their possible use as indicators of ecosystems health were analyzed. Plots 10 × 10 m with 4 replications were established to investigate three hypotheses: soil enzyme activities a) would increase with the restoration process; b) would be greater in surface soils than at lower depths; and c)would be correlated to selected physicochemical properties. Results showed that as the forests developed after restoration,invertase and peroxidase activities usually increased up to the 23 year point. Also soil enzyme activities were associated with surface soils and decreased with depths, suggesting that in earlier restoration stages surface addition of organic fertilizer to soils might be more effective than additions at depth. In the 0-20 cm soil, there were significant correlations (P ＜ 0.01 or ＜ 0.05) between some soil enzyme activities and some selected chemical properties. Therefore, temporal changes in enzyme activities should be included as an indicator when evaluating sustainable forest management practices.