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目的:探讨17β-雌二醇对谷氨酸诱导的SH-SY5Y细胞损伤的保护作用及可能机制。方法:选取SH-SY5Y细胞传代培养,分为四组:(1)阴性对照组;(2)氧化损伤组:0.1 mmol·L~(-1)谷氨酸作用24 h;(3)17β-雌二醇低、高浓度组:加入(1.0×10~(-4) mmol·L~(-1)和1.0×10~(-3)mmol·L~(-1))17β-雌二醇作用24 h之后,加入谷氨酸作用24 h。采用MTT比色法检测细胞存活率,流式细胞仪检测细胞活性氧(ROS)水平,Hoechst-PI染色观查细胞凋亡,分光光度计检测上清液中Caspase-3及Caspase-9含量。结果:7β-雌二醇能明显抑制谷氨酸诱导的细胞活性的下降,减少谷氨酸所致SH-SY5Y细胞内ROS的生成,降低细胞凋亡率,减少凋亡因子的活性。结论:17β-雌二醇对神经细胞损伤具有保护作用,这可能与其抗氧化作用有关。
Objective: To investigate the protective effect of 17β-estradiol on glutamate-induced SH-SY5Y cell injury and its possible mechanism. METHODS: SH-SY5Y cells were subcultured and divided into four groups: (1) negative control group; (2) oxidative damage group: 0.1 mmol·L -1 glutamate for 24 h; (3) 17β- Low estradiol and high estradiol groups were treated with 17β-estradiol (1.0 × 10 -4 mmol·L -1 and 1.0 × 10 -3 mmol·L -1) After 24 h, glutamate was added for 24 h. Cell viability was measured by MTT colorimetric assay. Cell viability was measured by flow cytometry. Apoptosis was detected by Hoechst-PI staining. The contents of Caspase-3 and Caspase-9 in supernatant were detected by spectrophotometer. Results: 7β-Estradiol significantly inhibited the decrease of glutamate-induced cell viability, decreased the production of ROS in SH-SY5Y cells induced by glutamate, decreased the apoptosis rate and decreased the activity of apoptotic factor. Conclusion: 17β-estradiol has a protective effect on nerve cell injury, which may be related to its antioxidant effect.