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为了探讨微生物多糖剂量效应在水产动物对不良环境刺激反应中的作用,本研究利用从南极冰海水中分离得到的南极细菌Pseudoalteromonas sp.3-3-1-2产生的胞外多糖,采用注射不同浓度的胞外多糖溶液的方式处理刺参,定期记录其生长状况并测定体腔液免疫相关指标的变化。结果表明,南极细菌胞外多糖处理后的刺参保护率升高,即较高浓度(4mg/mL)的细菌多糖可以有效降低刺参的死亡率。细菌多糖可以降低体腔液中MDA含量,虽然体腔液ACP、CAT和SOD活性在处理前期有所受到抑制,但是试验后期细菌多糖处理明显增强了上述免疫酶活性,以保护细胞免受氧自由基诱导的氧化损伤。处理过程中刺参体腔液的溶菌酶活性降低,但是细菌多糖的处理减弱了溶菌酶活性下降的幅度。此外,与2mg/mL处理组相比,4mg/mL处理组对刺参体腔液免疫酶的促进作用稍弱,但无显著差异。研究结果表明,极地细菌胞外多糖可对刺参产生一定的免疫保护作用,从而提高刺参逆境下的生长活力。
In order to investigate the role of microbial polysaccharide dose effect in aquatic animals on the adverse environmental stimuli response, the present study uses the Antarctic bacteria isolated from the Antarctic ice water Pseudoalteromonas sp.3-3-1-2 exopolysaccharide produced by different injection Concentration of polysaccharide solution of the treatment of sea cucumber, regular record of its growth and determination of body fluid immune-related indicators of change. The results showed that the protective rate of sea cucumber was increased after the extracellular polysaccharide of Antarctic bacteria was treated, that is, the bacterial polysaccharide of higher concentration (4mg / mL) could effectively reduce the mortality of sea cucumber. Bacterial polysaccharides can reduce the content of MDA in the body cavity fluid. Although ACP, CAT and SOD activity in the body cavity fluid were inhibited in the early stage of treatment, bacterial polysaccharide treatment at the end of the experiment significantly enhanced the above-mentioned immune enzyme activity to protect the cells from the induction of oxygen free radicals Oxidative damage. The lysozyme activity of the body fluid of Apostichopus japonicus decreased during the treatment, but the decrease of lysozyme activity was attenuated by bacterial polysaccharide treatment. In addition, the 4mg / mL treatment group was slightly weaker, but not significantly different, from the 2mg / mL treatment group. The results show that the polar bacteria exopolysaccharides may have some immunoprotective effect on sea cucumber, thus enhancing the growth vigor of sea cucumber under the stress of sea cucumber.