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用转PEPC、PPDK、NADP_ME、PEPC +PPDK酶基因水稻 (OryzasativaL .)及原种为材料 ,研究了光合作用对光照、温度、CO2 的响应和光抑制条件下的叶绿素荧光特性 ,结果如下 :1.转C4 光合酶基因水稻的饱和光合速率比原种高 ,其中转PEPC、PEPC +PPDK双基因水稻的光饱和点比原种高 2 0 0 μmol·m-2 ·s-1,饱和光合速率比原种分别高5 1.6 %和 5 8.5 % ;转PEPC基因水稻的羧化效率比原种高 49.3% ,CO2 补偿点降低 2 6 .2 % ;在高温 (35℃ )下 ,转PEPC基因水稻的光合速率比原种高 17.5 %。 2 .经光抑制处理 8d后 ,转PEPC、PEPC +PPDK酶基因水稻的PSⅡ光化学效率 (Fv/Fm)和光化学猝灭 (qP)下降 2 0 % - 30 % ,非光化学猝灭 (qN)增加了约 30 % ;但原种的Fv/Fm 和qP下降了 5 0 %多 ,qN变化不明显 ,表明转C4 光合基因水稻耐光抑制能力增强。这些结果为用生物技术提高水稻光合效率研究提供了新的依据和途径
The photosynthetic response to light, temperature, CO2 and chlorophyll fluorescence under photoinhibition conditions were studied using Oryzasativa L. and Oryza sativa L. cultivars transformed with PEPC, PPDK, NADP_ME and PEPC + PPDK genes. The results are as follows: 1. The saturation photosynthetic rate of transgenic rice with C4 photosynthetic enzyme was higher than that of the original plant, and the light saturation point of PEPC and PEPC + PPDK transgenic rice was 200 μmol · m-2 · s-1 higher than that of the original plant. The saturated photosynthetic rate The parental plants were 51.6% and 55.5% higher, respectively. The carboxylation efficiency of PEPC transgenic rice was 49.3% higher than that of the original plant and the CO2 compensation point was decreased by 26.2%. Under high temperature (35 ℃), the PEPC transgenic rice Photosynthetic rate 17.5% higher than the original species. The photochemical efficiency of PSⅡ (Fv / Fm) and photochemical quenching (qP) decreased by 20% -30% and non-photochemical quenching (qN) increased in PEPC and PEPC + PPDK transgenic rice plants after photopheresis treatment for 8 days About 30%. However, the Fv / Fm and qP of the original species decreased by more than 50% while the changes of qN did not change significantly. These results provide a new basis and approach for using biotechnology to improve the photosynthetic efficiency of rice