论文部分内容阅读
目的:建立特异、快速检测甲型副伤寒沙门菌的二重PCR法,应用于临床甲型副伤寒的快速诊断及流行病学溯源。方法:根据甲型副伤寒沙门菌1相鞭毛蛋白抗原(H1抗原)fliC-a基因及菌体抗原(O抗原)rfbS基因的核酸序列设计2对特异性引物,采用二重PCR法检测甲型副伤寒沙门菌标准株、非甲型副伤寒沙门菌标准株及甲型副伤寒沙门菌临床株。结果:实验结果证实,甲型副伤寒沙门菌标准株及临床株应用二重PCR方法均成功扩增出预先设计的2条特异性目的条带。结论:所建立的二重PCR法较之传统细菌检验方法具有快速、特异、敏感及简便之优势,可用于甲型副伤寒的早期诊断。
Objective: To establish a specific and rapid double-PCR method for the detection of Salmonella paratyphi A, which can be used in the rapid diagnosis and epidemiological exploration of clinical Paratyphoid. Methods: Two pairs of specific primers were designed according to the nucleotide sequence of the fliC-a gene of Salmonella paratyphi A (H1 antigen) and the rfbS gene of bacterial antigen (O antigen) Salmonella paratyphi standard strain, Salmonella paratyphi non-salmonella strain and Salmonella paratyphi A clinical strain. Results: The results of the experiment confirmed that two standard PCR bands of Salmonella paratyphi A and clinical strains were successfully amplified. Conclusion: The established duplex PCR method has the advantages of rapid, specific, sensitive and simple compared with the traditional bacterial test methods and can be used for the early diagnosis of paratyphoid fever.