论文部分内容阅读
本文报道一步超速离心分离VLDI、LDL、HDL和LDS的方法,该方法既不同于国外Havel的分步顺序超速离心法,又不同于国内DS沉淀合并密度梯度区带超速离心。只是利用区带头设计的优点,不用密度梯度液,仅将样品密度调高,这样可以避免长时间离心及使用KBr给脂蛋白带来的变化,分离样品量大、效果好、时间短、新鲜并具备生理功能,已成功地刚于脂蛋白受体研究达三年之久。
This paper reports a one-step ultracentrifugation VLDI, LDL, HDL and LDS method, which is different from the step-by-step ultracentrifugation Havel abroad, but also different from the domestic DS sedimentation density gradient zone ultracentrifugation. Just take advantage of the head design, without the use of density gradient solution, only the sample density increased, so you can avoid long-term centrifugation and the use of KBr lipoprotein to bring the change, large sample size, effective, time is short, fresh and With physiological functions, has been successfully just lipoprotein receptor research for three years.