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目的 研究开发简便、快速和有效的疟疾诊断技术。方法 利用噬菌体抗体库技术 ,在构建抗恶性疟原虫红内期噬菌体抗体库的基础上 ,经 3轮“吸附 -洗脱 -扩增”的富集反应后 ,筛选抗HRPII阳性克隆株并进行可溶性诱导表达 ,最后用ELISA和Westernblot等进行鉴定。结果 筛选出 6株抗HRPII阳性克隆株 ,表达的单链抗体Mr 为 310 0 0左右 ,能与HRPII抗原起特异性结合反应。结论 本研究为研制疟疾快速诊断试剂盒奠定了基础
Objective To study the development of a simple, rapid and effective diagnosis of malaria. Methods Based on phage antibody library construction, a phage antibody library of anti-Plasmodium falciparum was constructed. After 3 rounds of “adsorption-elution-amplification” enrichment reaction, anti-HRPII positive clones were screened for solubility Induced expression, and finally identified by ELISA and Westernblot. Results Six anti-HRPII positive clones were screened out. The expressed single chain antibody Mr was about 310 0 0 and could specifically bind to HRPII antigen. Conclusion This study laid the foundation for the rapid diagnosis of malaria