论文部分内容阅读
目的:观察非甾体类抗炎药消炎痛和阿斯匹林对C57BL/6 和Balb/c小鼠胃酸分泌的影响及胃黏膜损害的效应. 方法:应用幽门结扎术,测定消炎痛(40 mg/kg)和阿斯匹林(250 mg/kg)i.p后C57BL/6和Balb/c小鼠总胃酸分泌量(mL),胃酸度([H+]mmol/L)以及3 h总酸度(μmol/h)并应用Lanza score评分观察药物对胃黏膜的损害程度.同时应用[14C]-氨基比林蓄积试验观察2种小鼠离体胃对组胺刺激的效应. 结果:在基础状态下,C57BL/6和Balb/c小鼠的总胃酸分泌量和总酸度显著不同(0.82±0.06 vs 2.32±0.18; P<0.0 001;2.9±0.4 vs 9.7±1.6,P=0.0 005),而胃酸度没有显著差别(P=0.377).在C57BL/6小鼠,腹腔内应用消炎痛后总胃酸分泌量和总酸度与基础水平相比差别显著(2.08±0.16 vs 0.82±0.06,P<0.0 001; 9.4±1.1 vs 2.9±0.4,P<0.0 001),而胃酸度也没有显著差别(P=0.11);腹腔内应用阿斯匹林后总胃酸分泌量,胃酸度以及总酸度分别为1.5±0.3、16.1±1.4 和8.7±2.7,与基础水平相比均有显著差异(P=0.03, 0.009,0.03);在Balb/c小鼠,腹腔内应用消炎痛后总胃酸分泌量,胃酸度以及总酸度分别为3.4±0.3、5.8±1.0和6.5±1.2,前二者与基础水平相比有显著差异(P=0.007,0.01);腹腔内应用阿斯匹林后总胃酸分泌量,胃酸度以及总酸度与基础水平相比均没有显著差异(P=0.15,0.15,0.7).应用消炎痛后,C57BL/6和Balb/c小鼠Lanza score分别为12.6±0.9和16.4±1.1 (P=0.03);应用阿斯匹林后,C57BL/6和Balb/c小鼠Lanza score为10.4±1.0和11.0±1.1(P=0.91).应用[14C]-氨基比林蓄积试验观察2种小鼠离体胃对组胺刺激的效应,发现C57BL/6小鼠对不同浓度组胺刺激的效应显著低于Balb/c小鼠(10-5mol/L:30.8±8.5 vs 120.8±23.0.P=0.006;10-4mol/L:45.1±7.1 vs 236.1±48.7.P=0.005;10-3mol/L:37.9±5.3 vs 199.4±35.2,P=0.002). 结论:C57BL/6和Balb/c小鼠的胃酸分泌生理功能存在显著差异.消炎痛和阿斯匹林对C57BL/6和Balb/c小鼠的胃酸分泌功能和胃黏膜损害产生不同的效应.
Objective: To observe the effect of indomethacin and aspirin on gastric acid secretion and gastric mucosal lesion in C57BL / 6 and Balb / c mice.Methods: Pylorus ligation was used to determine the effect of indomethacin (40 total gastric acid secretion (mL), gastric acidity ([H +] mmol / L) and total acidity at 3 h (C57BL / 6 and Balb / c mice) following aspirin (250 mg / μmol / h), and the Lanza score score was used to observe the degree of gastric mucosal injury.At the same time, [14C] -aminopyrine buildup test was used to observe the effects of isolated stomach on histamine stimulation.Results: In the basal state , Total acid secretion and total acidity were significantly different in C57BL / 6 and Balb / c mice (0.82 ± 0.06 vs. 2.32 ± 0.18; P <0.0 001; 2.9 ± 0.4 vs. 9.7 ± 1.6, P = 0.0 005) (P = 0.377) .In C57BL / 6 mice, the total amount of gastric acid secretion and total acidity after intraperitoneal administration of indomethacin differed significantly from the basal level (2.08 ± 0.16 vs 0.82 ± 0.06, P <0.0 001 ; 9.4 ± 1.1 vs 2.9 ± 0.4, P <0.0 001), while there was no significant difference in gastric acidity (P = 0.11). The total gastric acid secretion, gastric acidity and total acidity after intraperitoneal aspirin administration were 1.5 ± 0.3, 16.1 ± 1.4 and 8. 7 ± 2.7, which were significantly different from the basal level (P = 0.03, 0.009, 0.03). In Balb / c mice, the total gastric acid secretion, gastric acidity and total acidity after intraperitoneal administration of indomethacin were 3.4 ± 0.3, 5.8 ± 1.0 and 6.5 ± 1.2 respectively. There was significant difference between the former two levels and the basal level (P = 0.007,0.01). The total gastric acid secretion, gastric acidity, (P = 0.15,0.15,0.7) .Lanza scores of C57BL / 6 and Balb / c mice were 12.6 ± 0.9 and 16.4 ± 1.1 (P = 0.03) after indomethacin treatment, The Lanza scores of C57BL / 6 and Balb / c mice were 10.4 ± 1.0 and 11.0 ± 1.1 (P = 0.91) after treatment with aspirin.Using [14C] -aminopyrine accumulation test, (10-5mol / L: 30.8 ± 8.5 vs 120.8 ± 23.0.P = 0.006; 10-4mol / L) in C57BL / 6 mice was significantly lower than that in Balb / c mice : 45.1 ± 7.1 vs 236.1 ± 48.7, P = 0.005; 10-3 mol / L: 37.9 ± 5.3 vs 199.4 ± 35.2, P = 0.002) .Conclusion: The gastric acid secretion physiological functions of C57BL / 6 and Balb / c mice are significant Differences in gastric acid score of indomethacin and aspirin in C57BL / 6 and Balb / c mice Function and gastric mucosal damage produced different effects.