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目的:口腔扁平苔藓(oral lichen planus,OLP)是一种发生于口腔黏膜的T淋巴细胞介导的自身免疫性疾病。辅助性T细胞(helper T lymphocytes,Th)在OLP的发病过程中具有重要作用,本研究旨在进一步探索OLP患者局部病损组织中辅助性T细胞亚群Th17细胞和Treg细胞的作用。方法:纳入43例OLP患者和13例健康志愿者。采用实时定量PCR法检测局部病损组织中Th17和Treg细胞的特征性转录因子RORγτ和FOXP3的表达,采用GraphPad Prism 5软件对其表达差异进行统计学分析。结果:OLP局部病损组织中转录因子RORγτ和FOXP3的表达显著高于正常黏膜组织,而且均与OLP的临床分型密切相关。萎缩糜烂型OLP组病损组织中RORγτ/FOXP3比值显著高于网状型OLP组和健康对照组,而网状型OLP组的RORγτ/FOXP3比值虽然高于对照组,但差异无显著性。结论:Th17细胞和Treg细胞均参与OLP的局部免疫反应;同时,Th17/Treg失衡也参与了重症型OLP的致病过程,且表现为Th17细胞优势。
OBJECTIVE: Oral lichen planus (OLP) is a T lymphocyte-mediated autoimmune disease that occurs in the oral mucosa. Helper T lymphocytes (Th) plays an important role in the pathogenesis of OLP. The purpose of this study is to further explore the role of Th17 and Treg cells in T lymphocyte subsets in local lesions in OLP patients. Methods: 43 OLP patients and 13 healthy volunteers were enrolled. Real-time quantitative PCR was used to detect the expression of RORγτ and FOXP3, which are characteristic transcription factors of Th17 and Treg cells in local lesions. Statistical analysis was performed using GraphPad Prism 5 software. Results: The expression of RORγτ and FOXP3 in OLP lesions was significantly higher than that in normal mucosa, and both were closely related to the clinical classification of OLP. The ratio of RORγτ / FOXP3 was significantly higher in the OLP group than in the OLP group and the healthy control group, while the RORγτ / FOXP3 ratio in the OLP group was higher than that in the control group, but the difference was not significant. CONCLUSION: Both Th17 cells and Treg cells are involved in the local immune response of OLP. Meanwhile, the imbalance of Th17 / Treg is also involved in the pathogenic process of severe OLP and shows the predominance of Th17 cells.