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1979年Boitieux等用抗体电极测定乙型肝炎表面抗原,据报道结果很好,因此我们试图建立起这一方法为检测微量抗原提供一种新的手段。装置抗体电极的一个重要步骤是将抗体用共价键偶联到明胶膜上。1982年我们曾报道过用叠氮法将抗体偶联在
In 1979, Boitieux et al. Measured HBsAg using antibody electrodes and reported good results. Therefore, we tried to establish this method to provide a new measure for the detection of trace antigens. An important step in assembling the antibody electrode is to conjugate the antibody to the gelatin film with a covalent bond. In 1982 we reported that the antibody was coupled by the azide method