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背景:及时、有效地控制轻症急性胰腺炎可防止其向重症急性胰腺炎演变,因而具有重要的临床价值。近年来,肝细胞生长因子(HGF)能否减轻啮齿动物的急性胰腺炎正日益受到关注。目的:观察外源性HGF能否减轻小鼠轻症急性胰腺炎,并探讨其作用机制。方法:以雨蛙肽腹腔注射诱发小鼠轻症急性胰腺炎,在制模前和制模中予各组小鼠皮下注射不同浓度的重组人HGF(rhHGF),部分小鼠同时皮下注射抗rhHGF单抗。根据血清淀粉酶、脂肪酶水平、胰腺组织学炎症评分和胰腺超微结构的变化评估炎症程度,并测定胰腺匀浆中花生四烯酸代谢产物血栓素(TX)B2和6鄄酮(keto)鄄前列腺素(PG)F1α的水平。结果:与单纯炎症组相比,给予4μg/kg或20μg/kgrhHGF小鼠的血清淀粉酶、脂肪酶水平和胰腺组织学炎症评分显著降低,胰腺匀浆中TXB2水平显著降低,6鄄keto鄄PGF1α水平显著增高,但4μg/kg与20μg/kgrhHGF的疗效无显著差异。给予rhHGF的同时给予抗rhHGF单抗,小鼠的上述各项参数与单纯炎症组无显著差异。结论:外源性HGF能减轻雨蛙肽诱导的小鼠轻症急性胰腺炎,其机制可能与对花生四烯酸代谢的影响有关。抗HGF单抗能抵消HGF的生物学作用。
Background: The timely and effective control of mild acute pancreatitis can prevent its evolution to severe acute pancreatitis and therefore has important clinical value. In recent years, whether hepatocyte growth factor (HGF) can reduce acute pancreatitis in rodents is receiving increasing attention. Objective: To observe whether exogenous HGF can alleviate mild acute pancreatitis in mice and to explore its mechanism. Methods: The mice were injected intraperitoneally with Hyla arboretum. The mice were injected intraperitoneally with recombinant human HGF (rhHGF) at different concentrations before and during the model making. Some mice were injected subcutaneously with anti-rhHGF anti. The degree of inflammation was evaluated according to changes of serum amylase, lipase level, pancreatic histological inflammatory score and pancreas ultrastructure. The arachidonic acid metabolites thromboxane B2 and keto were assayed in pancreatic homogenate, Prostaglandin (PG) F1α levels. Results: Serum amylase, lipase and pancreas histological inflammation score of 4μg / kg or 20μg / kg rhHGF mice were significantly lower than those of the simple inflammation group. TXB2 level in the pancreatic homogenate was significantly decreased, while 6-keto-PGF1α There was no significant difference in the effects of 4μg / kg and 20μg / kg rhHGF. RhHGF given at the same time anti-rhHGF mAb, the above parameters of mice and simple inflammation group no significant difference. Conclusion: Exogenous HGF can alleviate the mild acute pancreatitis induced by Hymenoptera in mice, and its mechanism may be related to the influence of arachidonic acid metabolism. Anti-HGF mAb can counteract the biological effects of HGF.