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[目的]探讨白藜芦醇对硝普钠诱导的软骨细胞凋亡及凋亡调控基因bax及bcl-2表达的影响。[方法]常规培养关节软骨细胞后,按不同浓度白藜芦醇(分别为25μmol/L、50μmol/L、100μmol/L)处理后分组并用硝普钠(SNP)(2.5 mmol/L)诱导细胞凋亡。采用MTT法检测各组软骨细胞活性、DAPI染色和流式细胞术检测各组细胞凋亡,采用western-blot技术检测各组细胞sirt1、bax、bcl-2的表达,RT-PCR法检测各组细胞sirt1mRNA的表达。[结果]随着白藜芦醇浓度的升高,MTT法结果显示处理后细胞活性依次增高;DAPI染色、流式细胞术检测显示处理后细胞凋亡相对较少;western-blot检测显示随着白藜芦醇浓度的升高,处理后细胞sirt1、bcl-2依次增高,而bax逐渐降低;RT-PCR法检测显示白藜芦醇处理后细胞sirt1 mRNA表达随浓度逐渐增高。[结论]白藜芦醇激活sirt1,使bcl-2/bax表达增高,是白藜芦醇抑制软骨细胞凋亡、预防骨关节炎的机制之一。
[Objective] To investigate the effects of resveratrol on sodium nitroprusside-induced chondrocyte apoptosis and the expression of bax and bcl-2. [Methods] Articular chondrocytes were cultured routinely and treated with different concentrations of resveratrol (25μmol / L, 50μmol / L, 100μmol / L, respectively) and then treated with sodium nitroprusside (SNP) Apoptosis. The activity of chondrocytes in each group was detected by MTT assay. The apoptosis of each group was detected by DAPI staining and flow cytometry. The expressions of sirt1, bax and bcl-2 were detected by western-blot and RT-PCR Cell sirt1 mRNA expression. [Results] With the increase of resveratrol concentration, MTT assay showed that the cell viability increased after treatment. DAPI staining and flow cytometry showed that apoptosis was relatively small after treatment; western-blot results showed that with the increase of resveratrol concentration, Resveratrol concentration increased, followed by treatment of sirt1, bcl-2 in turn increased, while bax decreased; RT-PCR assay showed that resveratrol cells sirt1 mRNA expression gradually increased with concentration. [Conclusion] Resveratrol activates sirt1 and increases the expression of bcl-2 / bax, which is one of the mechanisms of resveratrol in inhibiting chondrocyte apoptosis and preventing osteoarthritis.