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目的:考察理肺汤对慢性阻塞性肺疾病(COPD)大鼠肠道菌群和炎性因子的影响。方法:采用烟雾刺激+气管内滴注脂多糖(LPS)和臭氧暴露的方法建立COPD大鼠模型,分别以合生元、理肺汤灌胃,采集大鼠新鲜粪便,以选择性培养基培养为基础的活菌计数法进行肠杆菌、肠球菌、乳杆菌、双歧杆菌培养与计数。采用ELISA方法检测大鼠血清中白介素-16(IL-16),白介素-17(IL-17),C-反应蛋白(CRP)的含量。结果:与模型组相比,合生元组和理肺汤组均增加了大鼠体内共生菌,抑制了条件致病菌的繁殖。与模型组相比,IL-16、IL-17、CRP在正常组、合生元组及理肺汤组显著降低(P<0.05,P<0.01)。结论:理肺汤可能通过调节肠道菌群的结构,拮抗炎性反应,达到治疗COPD的效果。
Objective: To investigate the effects of LiFei Decoction on intestinal flora and inflammatory factors in rats with chronic obstructive pulmonary disease (COPD). Methods: COPD rat models were established by aerosol stimulation + endotracheal intratracheal instillation of lipopolysaccharide (LPS) and exposure to ozone. Fresh rat stools were collected by means of synbiotics and lianfei decoction, respectively. Fresh stools of rats were collected and cultured in selective medium The basis of viable count for Enterobacteriaceae, Enterococcus, Lactobacillus, Bifidobacterium culture and counting. Serum levels of IL-16, IL-17 and CRP were detected by ELISA. Results: Compared with the model group, the synbiotic group and the LiFei Tang group increased the commensal bacteria in the rat body and inhibited the reproduction of the pathogenic bacteria. Compared with model group, IL-16, IL-17 and CRP were significantly decreased in normal group, synbiotic group and LiFei Decoction group (P <0.05, P <0.01). Conclusion: LiFei Decoction can antagonize the inflammatory response by regulating the structure of intestinal flora and achieve the therapeutic effect on COPD.