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目的 观察分次外照射对小细胞肺癌NCI H4 4 6细胞系总RNA及其MDR1mRNA的影响。方法 采用GWGP80型远距离60 Co治疗机对进入指数生长期的NCI H4 4 6小细胞肺癌细胞系进行分次外照射 ,每次2Gy ,总剂量 5 0Gy。用Trizol分别提取未照射和已完成全部外照射剂量NCI H4 4 6细胞系的总RNA。通过RT PCR、琼脂胶电泳、Gelbase电脑软件计算电泳条带的平均吸光度A值 ,用两组细胞MDR1DNA与其 β actinDNA的平均吸光度A值的比确定两组细胞MDR1mRNA表达的高低。结果 在相同细胞数和相同体积的条件下 ,未照射组和照射组细胞总RNA的浓度分别为 2 5 .9μg/ml和 16 .6 μg/ml;未照射组细胞其MDR1DNA/ β actinDNA为1.0 78,照射组为 1.338。结论 对小细胞肺癌细胞系NCI H4 4 6进行外照射可抑制其总RNA的生物合成 ,同时可提高其MDR1mRNA的表达水平
Objective To observe the effect of fractionated external irradiation on total RNA and its MDR1 mRNA in small cell lung cancer NCI H4 4 6 cell line. Methods The GWI8080 long-range 60Co treatment machine was used to excise the NCI H4 4 small cell lung cancer cell line entering the exponential growth phase with a dose of 2 Gy each for a total dose of 50 Gy. Trizol was used to extract total RNA from unirradiated and completed external bolus doses of NCI H4 4 6 cell lines, respectively. The average absorbance A value of electrophoresis band was calculated by RT PCR, agarose gel electrophoresis and Gelbase computer software. The ratio of MDR1 mRNA to the average absorbance A value of β actinDNA was used to determine the expression of MDR1 mRNA in both groups. Results Under the same cell number and the same volume, the total RNA concentration in non-irradiated and irradiated cells was 25.9μg / ml and 16.6μg / ml, respectively. The non-irradiated cells had MDR1DNA / β actinDNA 1.0 78, the irradiation group was 1.338. Conclusion External beam irradiation of small cell lung cancer cell line NCI H4 4 6 can inhibit the biosynthesis of total RNA and increase the expression of MDR1 mRNA