论文部分内容阅读
目的 探索散发性大肠腺瘤细胞的原代培养 ;探讨环氧合酶 (COX) 2选择性抑制剂对大肠肿瘤防治的作用机制。方法 从人大肠腺瘤中分离培养上皮细胞 ;检测COX 2选择性抑制剂NS 398对细胞增殖的影响和大肠肿瘤中COX 2蛋白的表达。结果 取得大肠腺瘤 30枚 ,培养成功 2 3枚 ;NS 398对腺瘤细胞的抑制作用具有时间及剂量依存性 ;在大肠肿瘤中 ,COX 2蛋白表达显著增加 ,分别为 83 1%和 80 0 %。结论 避免污染、恰当的消化时间、合适的细胞数和成纤维细胞的去除 ,是大肠腺瘤细胞原代培养成功的关键 ;NS 398对细胞生长具有明显抑制作用 ;COX 2蛋白在大肠肿瘤形成的早期阶段出现表达增强
Objective To explore the primary culture of sporadic colorectal adenoma cells and to explore the mechanism of cyclooxygenase (COX) 2 selective inhibitors in the prevention and treatment of colorectal tumors. Methods Epithelial cells were isolated and cultured from human colorectal adenomas. The effects of COX 2 selective inhibitor NS 398 on cell proliferation and COX 2 protein expression in colorectal tumors were detected. Results Thirty colorectal adenomas were obtained, and 23 were successfully cultured. The inhibitory effect of NS 398 on adenoma cells was time- and dose-dependent. In colorectal tumors, the expression of COX 2 protein was significantly increased, which was 83 1% and 80 0, respectively. %. Conclusion Avoiding contamination, proper digestion time, proper cell number and removal of fibroblasts are the key to successful primary culture of colorectal adenoma cells; NS 398 has a significant inhibitory effect on cell growth; COX 2 protein is formed in large intestine tumors Increased expression in the early stages