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目的了解不同鱼种对壬基酚的毒性差异。方法选择红鲫、草鱼和鲢鱼鱼苗,4-壬基酚换水式染毒进行96h急性毒性试验、12周亚慢性毒性试验、外周血红细胞微核试验,高效液相色谱法测定组织中4-壬基酚。结果①4-壬基酚对红鲫、草鱼和鲢鱼的96h LC50分别为251.30、155.84和187.01μg/L。②染毒12周,红鲫高剂量组肝胰脏系数降低,中、高剂量组卵巢系数增高(P<0.05);草鱼中剂量组肾脏系数降低(P<0.05);鲢鱼高剂量组肝胰脏系数降低(P<0.05)。③染毒4周时,红鲫和鲢鱼中、高剂量组及草鱼高剂量组微核细胞率与对照组相比显著增高(P<0.05);染毒8周及以后,受试鱼各剂量组微核细胞率均增高(P<0.05);④受试鱼肌肉、脑和肝胰脏等组织样品中均有4-壬基酚检出,其中肝胰脏的生物浓缩系数最高;红鲫对4-壬基酚的生物浓缩系数高于草鱼和鲢鱼。结论红鲫对壬基酚较敏感。
Objective To understand the toxic effects of different species on nonylphenol. Methods Red crucian carp (Carassius auratus), grass carp and silver carp (Hypophthalmichthys molitrix) fish fry were selected for 4-nonylphenol water for 96 h acute toxicity test, 12 weeks subchronic toxicity test, peripheral blood erythrocyte micronucleus test and high performance liquid chromatography Nonylphenol. Results ① 96h LC50 of 4-nonylphenol on crucian carp, grass carp and silver carp were 251.30, 155.84 and 187.01 μg / L, respectively. ② After 12 weeks of treatment, the coefficient of hepatopancreas was decreased in the high-dose red crucian carp and the ovarian index was increased in the medium and high dose groups (P <0.05). The renal coefficient of middle-dose grass carp group was lower (P <0.05) Pancreas coefficient decreased (P <0.05). ③ At 4 weeks of exposure, the micronuclei in high-dose group and high-dose grass carp group were significantly higher than those in control group (P <0.05). After 8 and 8 weeks of exposure, (P <0.05) .4-Nonylphenol was detected in muscle, brain, hepatopancreas and other tissues of the test fish, and the bioconcentration factor of hepatopancreas was the highest. The red Crucian carp 4-nonylphenol bioconcentration factor higher than grass carp and silver carp. Conclusion Red crucian is sensitive to nonylphenol.