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一、目的、材料与方法 多效唑(PP_(333))是近年来研究使用的一种生长抑制剂,它除拮抗赤霉素作用外,还具有多种效能。本试验试图了解PP_(333)对试管苗生根与移栽成活率的影响。 以菊花(Dendranthema morifolium Tzvel.)和蔓长春花(Vinca major L.)之健壮试管苗为材料。MS培养基为基本培养基,添加不同浓度的PP_(333)(0.01～50ppm),蔗糖2％,琼脂0.8％,pH5.8。培养基采用高压蒸气法灭菌。培养温度为25±2℃,光照10h/d。培养2周后观察记录。 二、结果与分析 研究表明,适当浓度的PP_(333)对菊花和蔓长春花试管苗生根具有明显的促进作用。当PP_(333)浓度为0.01～1.0ppm时,菊花苗生根率为100％;蔓长春花的生根率随PP_(333)浓度增
First, the purpose, materials and methods Paclobutrazol (PP_ (333)) is a growth inhibitor studied in recent years, in addition to antagonizing the role of gibberellin, but also has a variety of efficacy. This experiment attempted to understand the effect of PP_ (333) on the survival rate of rooting and transplanting of in vitro plantlets. The robust plantlets of Dendranthema morifolium Tzvel. And Vinca major L. were used as materials. MS medium supplemented with different concentrations of PP_ (333) (0.01 ~ 50ppm), sucrose 2%, agar 0.8%, pH5.8. The medium is autoclaved. Incubation temperature 25 ± 2 ℃, light 10h / d. After 2 weeks of observation record. Second, the results and analysis Studies have shown that appropriate concentrations of PP_ (333) on the chrysanthemum and vinca vulgaris root canal test has a significant role in promoting. When the concentration of PP_ (333) was 0.01 ~ 1.0ppm, the rooting rate of chrysanthemum seedlings was 100%. The rooting rate of vinca sativa increased with the increase of PP_ (333)