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目的:探讨水飞蓟素对链脲菌素-尼克酰胺诱导的2型糖尿病肾病大鼠肾脏的保护作用。方法:8周龄雄性albino Wistar大鼠60只,随机选取45只,采用肌注链脲菌素-尼克酰胺复制2型糖尿病模型(其中3只大鼠复制模型失败),随即分为糖尿病对照组(DC组,n=14)、糖尿病水飞蓟素低剂量治疗组(DT-L,60 mg·kg-1·d-1,n=14)和糖尿病水飞蓟素高剂量治疗组(DT-H,120 mg·kg-1·d-1,n=14)。其余15只大鼠仅注射等体积的缓冲液作为正常对照组(NC,n=15)。模型成功后1周给予水飞蓟素灌胃治疗,持续60 d。采用半自动生化分析仪检测糖化血红蛋白、尿量、血清肌酐、血清尿酸、尿白蛋白水平等生化指标。结果:实验结束时,与NC大鼠比较,DC大鼠的血糖、糖化血红蛋白、尿量、血清尿酸、尿白蛋白等水平均升高(P<0.05)。与DC大鼠比较较,DT-L和DT-H大鼠表现出较低水平的血糖、糖化血红蛋白、尿量、血清肌酐、血清尿酸、尿白蛋白水平,差异有统计学意义(P<0.05)。结论:水飞蓟素对2型糖尿病大鼠的肾脏具有保护作用,为其临床应用奠定实验基础。
Objective: To investigate the protective effects of silymarin on the kidney of streptozotocin-nicotinamide induced type 2 diabetic nephropathy rats. Methods: Sixty albino Wistar rats aged 8 weeks were randomly divided into diabetic group (n = 20) and model group (n = 45). The model of type 2 diabetes was reproduced by intramuscular injection of streptozotocin-nicotinamide (3 of them failed to replicate the model) (DT-H, 120 mg · kg-1 · d-1, n = 14) and diabetic high-dose silymarin treatment group (DC group, n = 14) kg-1 · d-1, n = 14). The remaining 15 rats were injected only with an equal volume of buffer as a normal control (NC, n = 15). One week after the model was given, silymarin was given intragastrically for 60 days. Semi-automatic biochemical analyzer was used to detect biochemical indexes such as glycated hemoglobin, urine output, serum creatinine, serum uric acid and urine albumin. Results: At the end of the experiment, the levels of blood glucose, glycosylated hemoglobin, urine volume, serum uric acid and urinary albumin in DC rats were significantly higher than those in NC rats (P <0.05). Compared with DC rats, DT-L and DT-H rats showed a lower level of blood glucose, glycosylated hemoglobin, urine output, serum creatinine, serum uric acid, urinary albumin levels, the difference was statistically significant (P <0.05 ). Conclusion: Silymarin has a protective effect on the kidneys of type 2 diabetic rats, laying an experimental foundation for its clinical application.