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HPD光敏法有希望成为一种新型诊断、治疗癌症的方法,近年发展很快。但目前为临床诊治提供科学依据的基础研究尚待加强。而基础研究的大量工作是以离体培养细胞为对象的。为与培养细胞的过程相适应,对照射光源提出了一些特殊要求。潴留在组织内的HPD荧光带内波长的光都会引起光敏作用达到杀伤组织的效果,因此可利用带滤光设备的连续谱光源。所研制的照射光源由钨灯加两面介质膜镜进行带通滤波而构成。其输出波长主要范围(600—700)nm区,而在630nm及690nm处相对强度为94%及56%％;光斑大于50mm;均匀性较好;功率合适(最大功率密度125mw/cm~2);可作垂直及水平方向照射(两方向均可移动);具有结构简单,操作维修方便,寿命长,价廉等优点。我们用它与He—Ne激光器在输出功率密度相同的条件下照射HPD处理的MGC80-3细胞比较其影响,结果对细胞损害相同。并用它作了多种癌细胞光敏效应的测定及比较等多项基础研究工作。均得到较为满意的结果。
HPD photosensitive method hopeful to become a new diagnosis, treatment of cancer, rapid development in recent years. However, the basic research that provides a scientific basis for clinical diagnosis and treatment needs to be strengthened. A large number of basic research work is based on in vitro cultured cells for the object. In order to adapt to the process of cultivating cells, some special requirements are put forward for the illuminating light source. Light trapped within the HPD fluorescent band within the tissue will cause photosensitization to kill the tissue, thus allowing the use of a continuous spectrum light source with a filter. The developed light source by the tungsten lamp plus two dielectric mirror bandpass filter formed. The output wavelength of the main range (600-700) nm area, and at 630nm and 690nm relative intensity of 94% and 56 %%; spot is greater than 50mm; better uniformity; power is appropriate (maximum power density 125mw / cm2) ; Can be used for vertical and horizontal irradiation (both directions can be moved); with simple structure, easy operation and maintenance, long life, low cost and so on. We compared the effect of HPD-treated MGC80-3 cells with He-Ne laser at the same output power density, and the result was the same as the cell damage. And use it for a variety of cancer cells photosensitivity determination and comparison of a number of basic research work. Have been more satisfied with the results.